Carbenoxolone disodium
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Carbenoxolone disodium(甘珀酸二钠)是11β-羟基类固醇脱氢酶(11β-HSD)的抑制剂[1]。
11β-HSD是一个酶家族,参与调节糖皮质激素进入类固醇受体。
在牛主动脉内皮细胞(BAEC)中,Carbenoxolone disodium能阻断间隙连接通讯(GJC),调节Cx43的表达,而Cx43的表达基于连接的开放[2]。
Carbenoxolone disodium是11β-HSD的抑制剂,可引起低钾血症和高钠血症。在雄性大鼠中,腹腔注射CX(100 mg/kg)1小时后,抑制了肝、肾、垂体、海马、丘脑和杏仁核中的11β-HSD。第三脑室注射CX(1.5 mg/kg),抑制了海马和垂体中11-HSD催化的皮质酮向11-dehydrocorticosterone的转变,产生多动行为,对于肝脏或肾脏没有影响。脑室内注射CX(10-50ug/rat)抑制下丘脑和海马的11β-HSD [1]。这些结果表明,低剂量的CX可以改变特定脑区11β-HSD的活性而不影响其在外周组织中的活性,在垂体中只有轻微的活性,这些为研究该酶的关键作用提供了方法。
参考文献:
[1]. Jellinck PH, Monder C, McEwen BS, et al. Differential inhibition of 11 beta-hydroxysteroid dehydrogenase by carbenoxolone in rat brain regions and peripheral tissues. J Steroid Biochem Mol Biol, 1993, 46(2): 209-213.
[2]. Sagar GD, Larson DM. Carbenoxolone inhibits junctional trasnfer and upregulates connexin43 expression by a protein kinase A-dependent pathway. J Cell Biochem, 2006, 98(6): 1543-1551
- 1. Rotenberg MY, Yamamoto N, et al. "Living myofibroblast-silicon composites for probing electrical coupling in cardiac systems." Proc Natl Acad Sci U S A. 2019 Oct 17. pii: 201913651. PMID:31624124
- 2. Zhou L, Liu C, et al. "Pannexin-1 is involved in neuronal apoptosis and degeneration in experimental intracerebral hemorrhage in rats." Mol Med Rep. 2018 Apr;17(4):5684-5691. PMID:29484398
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 614.72 |
Cas No. | 7421-40-1 |
Formula | C34H48Na2O7 |
Solubility | ≥30.74 mg/mL in DMSO; ≥39.1 mg/mL in EtOH; ≥55.1 mg/mL in H2O |
Chemical Name | disodium;10-(3-carboxylatopropanoyloxy)-2,4a,6a,6b,9,9,12a-heptamethyl-13-oxo-3,4,5,6,6a,7,8,8a,10,11,12,14b-dodecahydro-1H-picene-2-carboxylate |
SDF | Download SDF |
Canonical SMILES | CC1(C2CCC3(C(C2(CCC1OC(=O)CCC(=O)[O-])C)C(=O)C=C4C3(CCC5(C4CC(CC5)(C)C(=O)[O-])C)C)C)C.[Na+].[Na+] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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