Calpain Inhibitor II, ALLM
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Calpain inhibitor II（CPI-2）是一种细胞通透性的钙蛋白酶I、钙蛋白酶II、组织蛋白酶L和组织蛋白酶B抑制剂。
在急性淋巴细胞白血病（ALL：ALL-1、RS4;11和JURKAT）和非霍奇金淋巴瘤（NHL：RAMOS和DAUDI）细胞系中，通过MC540单荧光测量，calpain inhibitor II在50或100 μM浓度时诱导细胞凋亡。此外，研究表明，calpain inhibitor II诱导细胞凋亡不需要BTK或LYN的参与。Calpain inhibitor II对钙蛋白酶的抑制可诱导促进细胞凋亡的caspase系统[1]。与calpain inhibitor I不同，calpain inhibitor II 不能抑制NF-κB和使DLD1-TRAIL/R细胞对TRAIL蛋白敏感[2]。
参考文献：
[1] Zhu DM1, Uckun FM. Calpain inhibitor II induces caspase-dependent apoptosis in human acute lymphoblastic leukemia and non-Hodgkin's lymphoma cells as well as some solid tumor cells. Clin Cancer Res. 2000 Jun;6(6):2456-63.
[2] Zhu H1, Zhang L, Huang X, Davis JJ, Jacob DA, Teraishi F, Chiao P, Fang B. Overcoming acquired resistance to TRAIL by chemotherapeutic agents and calpain inhibitor I through distinct mechanisms. Mol Ther. 2004 May;9(5):666-73.
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- 2. Ednie AR, Parrish AR, et al. "Reduced hybrid/complex N-glycosylation disrupts cardiac electrical signaling and calcium handling in a model of dilated cardiomyopathy." J Mol Cell Cardiol. 2019 Jul;132:13-23. PMID: 31071333
- 3. Li W, Liu BD, et al. "Alteration of Androgen Receptor Protein Stability by Triptolide in LNCaP Cells." Medicina (Kaunas). 2018 May 30;54(3). pii: E39. PMID: 30344270
- 4. Chen Y, Yu Y, et al. "Bradykinin promotes migration and invasion of hepatocellular carcinoma cells through TRPM7 and MMP2." Exp Cell Res. 2016 Sep 29. PMID: 27693494
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 401.57 |
| Cas No. | 136632-32-1 |
| Formula | C19H35N3O4S |
| Synonyms | N-Acetyl-Leu-Leu-Methional |
| Solubility | insoluble in H2O; ≥14.85 mg/mL in DMSO; ≥20.27 mg/mL in EtOH |
| Chemical Name | 2-acetamido-4-methyl-N-[4-methyl-1-[(4-methylsulfanyl-1-oxobutan-2-yl)amino]-1-oxopentan-2-yl]pentanamide |
| SDF | Download SDF |
| Canonical SMILES | CC(C)CC(C(=O)NC(CC(C)C)C(=O)NC(CCSC)C=O)NC(=O)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
|
细胞系 |
急性淋巴细胞白血病（ALL）细胞系（ALL-1、RS4;11和JURKAT）和非何杰金氏淋巴瘤（NHL）细胞系（RAMOS和DAUDI） |
|
制备方法 |
在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液，一般步骤如下：请将试管置于37℃加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20℃可放置数月。 |
|
反应条件 |
50或100 μM；24小时 |
|
实验结果 |
当剂量为50或100 μM时，Calpain inhibitor II诱导ALL（ALL-1、RS4;11和JURKAT）和非霍奇金淋巴瘤（RAMOS和DAUDI）细胞系凋亡。此外，研究表明，Calpain inhibitor II不需要BTK和LYN诱导细胞凋亡。Calpain inhibitor II通过抑制钙蛋白酶激活促凋亡蛋白酶系统。 |
|
References: [1]. Zhu DM1, Uckun FM. Calpain inhibitor II induces caspase-dependent apoptosis in human acute lymphoblastic leukemia and non-Hodgkin's lymphoma cells as well as some solid tumor cells. Clin Cancer Res. 2000 Jun;6(6):2456-63. |
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| 描述 | Calpain Inhibitor II, ALLM是一种细胞通透性的钙蛋白酶I、钙蛋白酶II、组织蛋白酶L（cathepsin L）和组织蛋白酶B（cathepsin B）抑制剂，Ki值分别为120 nM、230 nM、0.6 nM和100 nM。 | |||||
| 靶点 | calpain I | calpain II | cathepsin L | cathepsin B | ||
| IC50 | 120 nM (Ki) | 230 nM (Ki) | 0.6 nM (Ki) | 100 nM (Ki) | ||
质量控制和MSDS
- 批次:
化学结构
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