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Calcitriol

现货
Catalog No.
B2141
维生素D3的活性代谢物
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 3,500.00
现货
1mg
¥ 640.00
现货
10mg
¥ 4,080.00
现货
25mg
¥ 5,670.00
现货
100mg
¥ 19,860.00
现货
500mg
¥ 44,680.00
Ship with 10-15 days

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Background

Calcitriol is a form of Vitamin D, is converted to metabolites more potent and rapidly acting than other forms of Vitamin D [1].

Calcitriol has played an important role in mineral and skeletal homeostasis by regulating the differentiation, growth and the function of the cell immune system. In vitro studies, Calcitriol has been reported to dose-dependently inhibit the production of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) in human peripheral blood cells (PBMC) stimulated by LPS. Furthermore, Calcitriol has been revealed to significantly reduced the basal intradialytic increases of TNF-αand IL-1βin vivo studies. Apart from these, Calcitriol has also been reported to modulate the cytokine production indirectly through the effect on calcium and PTH metabolism [2].

References:
[1] Holick MF, Schnoes HK, DeLuca HF. Identification of 1,25-dihydroxycholecalciferol, a form of vitamin D3 metabolically active in the intestine. Proc Natl Acad Sci U S A. 1971 Apr;68(4):803-4.
[2] Panichi V1, De Pietro S, Andreini B, Bianchi AM, Migliori M, Taccola D, Giovannini L, Tetta C, Palla R. Calcitriol modulates in vivo and in vitro cytokine production: a role for intracellular calcium. Kidney Int. 1998 Nov;54(5):1463-9.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt416.64
Cas No.32222-06-3
FormulaC27H44O3
Solubility≥20.832mg/mL in DMSO
Chemical Name(1R,3S,5Z)-5-[(2E)-2-[(1R,3aS,7aR)-1-[(2R)-6-hydroxy-6-methylheptan-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexane-1,3-diol
SDFDownload SDF
Canonical SMILESCC(CCCC(C)(C)O)C1CCC2C1(CCCC2=CC=C3CC(CC(C3=C)O)O)C
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

细胞实验[1]:

细胞系

基底细胞癌(BBC)细胞系ASZ001

溶解方法

在DMSO中的溶解度大于20.8mg/mL。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。

反应条件

10 nM,48 h

应用

在BCC ASZ001细胞中,calcitriol显著抑制Hh信号通路,激活Vdr信号通路。Calcitriol抑制细胞增殖,但是caspase 3/7的活性没有变化,所以其对凋亡没有影响。

临床实验[2]:

临床样本

11-35岁的近期发病1型糖尿病病人

剂量

每天0.25 μg,持续2年

应用

在6,12和24个月,calcitriol组和安慰剂组的A1C,C肽和胰岛素需求值没有区别。这些结果表明,所使用剂量的calcitriol不能保护具有高C肽的近期发病1型糖尿病病人的β细胞功能。

注意事项

请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的,属于正常现象。

References:

[1]. Uhmann A, Niemann H, Lammering B, et al. Antitumoral effects of calcitriol in basal cell carcinomas involve inhibition of hedgehog signaling and induction of vitamin D receptor signaling and differentiation[J]. Molecular cancer therapeutics, 2011, 10(11): 2179-2188.

[2]. Bizzarri C, Pitocco D, Napoli N, et al. No Protective Effect of Calcitriol on β-Cell Function in Recent-Onset Type 1 Diabetes[J]. Diabetes care, 2010, 33(9): 1962-1963.

质量控制

化学结构

Calcitriol