BW 755C
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 0.75 μM, 0.65 μg/ml, and 1.2 μg/ml for 5-LO, COX-1, and COX-2, respectively
BW 755C is a dual inhibitor of 5-lipoxygenase (5-LO) and cyclooxygenase (COX) pathways.
Constitutive cyclooxygenase (COX-1) is present in cells under physiological conditions, whereas COX-2 is induced by some cytokines, mitogens, and endotoxin in pathological conditions, such as inflammation. Since 5-lipoxygenase (5-LO) oxidizes arachidonic acid to 5-hydroperoxyeicosatetraenoic acid in the first step of the leukotriene pathway, 5-LO inhibitors should prevent leukotriene biosynthesis and thus prove useful in the treatment of allergic asthma.
In vitro: Previous study found that BW 755C and other nonsteroidal antiinflammatory drugs including diclofenac, acetaminophen, and naproxen showed approximately equipotent inhibitory effects on COX-1 and COX-2 in intact cells. Whereas, BF 389 was the most potent and most selective inhibitor of COX-2 in intact cells [1].
In vivo: An animal study was conducted to examine whether BW-755C delayed neuronal death in the hippocampal CA1 sector in Mongolian gerbils after 5 minutes of forebrain ischemia. Gerbils were injected with BW-755C. Seven days after ischemic insult, the animals were perfusion-fixed, and the neuronal density in the hippocampal CA, sector was estimated. Results showed that in ischemic gerbils with vehicle administration, the average neuronal density was 13 for BW-755C. In ischemic gerbils treated with 30 mg/kg BW-755C, the average neuronal densities was 14 [2].
Clinical trial: So far, no clinical study has been conducted.
References:
[1] Mitchell, J. A.,Akarasereenont, P.,Thiemermann, C., et al. Selectivity of nonsteroidal antiinflammatory drugs as inhibitors of constitutive and inducible cyclooxygenase. Proceedings of the National Academy of Sciences of the United States of America 90, 11693-11697 (1993).
[2] Nakagomi T, Sasaki T, Kirino T, Tamura A, Noguchi M, Saito I, Takakura K. Effect of cyclooxygenase and lipoxygenase inhibitors on delayed neuronal death in the gerbil hippocampus. Stroke. 1989 Jul;20(7):925-9.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 229.3 |
Cas No. | 66000-40-6 |
Formula | C10H10F3N3 |
Solubility | ≤30mg/ml in ethanol;30mg/ml in DMSO;25mg/ml in dimethyl formamide |
Chemical Name | 4,5-dihydro-1-[3-(trifluoromethyl)phenyl]-1H-pyrazol-3-amine |
SDF | Download SDF |
Canonical SMILES | NC(CC1)=NN1C2=CC=CC(C(F)(F)F)=C2 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |