Butein
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Butein是一种具有抗氧化作用的查尔酮，具有多种药理作用。
活性氧（ROS）在细胞内通过多种机制产生，依赖于细胞和组织类型，主要通过细胞膜、线粒体、过氧化物酶体和内质网的ROS NADPH氧化酶（NOX）复合体而产生，可由牙科粘合漂白剂和牙髓病诱导产生，ROS可激活氧化应激。[1]ROS是正常氧代谢的天然产物，在细胞信号和内稳态中具有重要作用。但是，在多次环境压力后，ROS水平急剧增加。[2] 在butein 存在时，H2O2诱发的细胞毒性和ROS生成会被阻断，这一效果是剂量依赖的。由于ROS的双重作用，基于促氧化和抗氧化功能的抗癌制剂都被研发出来。
Butein可诱导B16黑色素瘤细胞和人早幼粒白血病细胞凋亡，抑制糖尿病并发症，并能抑制诸如蛋白激酶和谷胱甘肽还原酶等酶类。[3，4，5，6]最近，Butein从R. verniciflua中分离出来，通过JNK–Nrf2/ARE依赖的HO-1表达，抑制HDP细胞中H2O2引起的氧化性细胞损伤。[7]另外，体外流式细胞仪分析和RT-PCR表明，Butein可通过抑制NF-kB活性，减弱VEGF和MMP-9的活性。并且，通过电泳迁移率变动分析（EMSA）和酶联免疫吸附试验（ELISA）发现，Butein可抑制肿瘤坏死因子ɑ和佛波醇-12-肉豆蔻酸酯-13-乙酸酯诱导的VEGF和MMP-9表达，最终导致前列腺癌细胞生长、侵袭和血管生成的抑制。[8]因此，Butein有希望成为治疗多种牙科疾病的制剂。
参考文献：
1. Han D, Williams E, Cadenas E."Mitochondrial respiratory chain-dependent generation of superoxide anion and its release into the intermembrane space". Biochem. J. 2010, 353 (2): 411-6.
2. Devasagayam, TPA; Tilak JC; Boloor KK; Sane Ketaki S; Ghaskadbi Saroj S; Lele RD. "Free Radicals and Antioxidants in Human Health: Current Status and Future Prospects". Journal of Association of Physicians of India (JAPI), 2004, 52: 796.
3. S. Sogawa, Y. Nero, H. Ueda, T. Miki. Protective effects of hydroxychalcones on free radical-induced cell damage. Biol. Pharm. Bull., 1994, 251-256.
4. J.C. Lee, K.T. Lim, Y.S. Jang. Identification of Rhus verniciflua Stokes compounds that exhibit free radical scavenging and anti-apoptotic properties. Biochim. Biophys. Acta, 2002, 181-191.
5. S.M. Yu, Z.J. Cheng, S.C. Kuo. Endothelium-dependent relaxation of rat aorta by butein, a novel cyclic AMP-specific phosphodiesterase inhibitor. Eur. J. Pharmacol., 1995, 69-77.
6. K. Iwashita, M. Kobori, K. Yamaki, T. Tsushida. Flavonoids inhibit cell growth and induce apoptosis in B16 melanoma 4A5 cells. Biosci. Biotechnol. Biochem., 2000, 1813-1820.
7. Dong-Sung Lee.et al. Butein protects human dental pulp cells from hydrogen peroxide-induced oxidative toxicity via Nrf2 pathway-dependent heme oxygenase-1 expressions.Toxicology in Vitro, 2013, 874-881.
8. Dong-Oh Moona.et al. Butein suppresses the expression of nuclear factor-kappa B-mediated matrix metalloproteinase-9 and vascular endothelial growth factor in prostate cancer cells. Toxicology in Vitro, 2010,24(7) ,1927-1934.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 272.25 |
| Cas No. | 487-52-5 |
| Formula | C15H12O5 |
| Solubility | insoluble in H2O; ≥13.6 mg/mL in DMSO; ≥8.86 mg/mL in EtOH with ultrasonic |
| Chemical Name | (E)-1-(2,4-dihydroxyphenyl)-3-(3,4-dihydroxyphenyl)prop-2-en-1-one |
| SDF | Download SDF |
| Canonical SMILES | OC1=C(C=CC(O)=C1)C(/C=C/C2=CC(O)=C(O)C=C2)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | Butein是5-脂加氧酶、乙酰脂肪酰蛋白还原酶（P. falciparum）、血管紧张素转化酶和Src激酶的抑制剂，Ki值分别为0.01、2.97、0.73和65 μM。 | |||||
| 靶点 | 5-lipoxygenase | enoyl-acyl-carrier protein reductase (P. falciparum) | angiotensin-converting enzyme | Src kinase | ||
| IC50 | 0.01 μM (Ki) | 2.97 μM (Ki) | 0.73 μM (Ki) | 65 μM (Ki) | ||
化学结构
