Bryostatin 1
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Bryostatin 1 is an activator of protein kinase C (PKC) [1].
PKC is a monomeric Ca2+- and phospholipid-dependent Ser/Thr protein kinase and plays an important role in growth factor-activated signaling and malignant transformation.
Bryostatin 1 is a PKC activator. In 3T3 cells, bryostatin 1 stimulated ornithine decarboxylase (ODC) activity in a dose-dependent way [1]. In HL-60 cells, bryostatin 1 induced protein kinase C translocated from the cytosolic to membrane. Bryostatin 1 (10 nM) induced monocytic differentiation and partially inhibited proliferation. Also, bryostatin 1 (10 nM) reduced the expression of c-myc and induced c-fos, c-fms and tumor necrosis factor transcripts [2].
In SENCAR mouse, bryostatin 1 caused [3H]TdR incorporation into epidermal DNA and induced epidermal hyperplasia in a dose-dependent way. Also, bryostatin 1 stimulated alkaline phosphatase activity in the epidermis and significantly reduced the tumor incidence [1]. In a B16 melanoma pulmonary metastases mice model, bryostatin 1 significantly reduced the number of lung nodules. Bryostatin 1 inhibited the generation of interleukin 2 by lymphokine-activated killer cells and also inhibited natural killer cell activity [3].
References:
[1]. Gschwendt M, Fürstenberger G, Rose-John S, et al. Bryostatin 1, an activator of protein kinase C, mimics as well as inhibits biological effects of the phorbol ester TPA in vivo and in vitro. Carcinogenesis, 1988, 9(4): 555-562.
[2]. Stone RM, Sariban E, Pettit GR, et al. Bryostatin 1 activates protein kinase C and induces monocytic differentiation of HL-60 cells. Blood, 1988, 72(1): 208-213.
[3]. Schuchter LM, Esa AH, May S, et al. Successful treatment of murine melanoma with bryostatin 1. Cancer Res, 1991, 51(2): 682-687.
Physical Appearance | White lyophilised solid |
Storage | Store at -20°C |
M.Wt | 905.04 |
Cas No. | 83314-01-6 |
Formula | C47H68O17 |
Solubility | Soluble in DMSO and in ethanol |
SDF | Download SDF |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |