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Brassinolide

现货
Catalog No.
A3265
植物生长调节剂
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 900.00
现货
5mg
¥ 440.00
现货
10mg
¥ 670.00
现货

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Background

IC50: N/A

Brassinolide is a plant growth regulator. Plant growth regulators are naturally produced by plants and are critical for regulating their own growth. Plant growth regulators act via modifying or controlling plant growth processes, such as leave and flower formation, stem elongation, as well as fruit development and ripening.

In vitro: Brassinolide induced the time and concentration-dependent cytotoxicity in PC-3 cells. The mode of such cell death appeared to be apoptosis predominately, as demonstrated by fluorescence, flow-cytometric analyses and transmission electron microscopes. Moreover, Caspase-3 activity was increased after brassinolide treatment obviously. Western blot studies showed brassinolide treatment triggered a time-dependent decrease in the expression of Bcl-2 [1].

In vivo: Brassinolide had the similar function of reducing the blood glucose levels as phenformin, but without the dose-dependent manner. The blood glucose levels showed significant differences after brassinolide treatment with different doses (200, 100, and 50 mg/kg). These results indicated that brassinolide could reduce the blood glucose levels without toxicity [2].

Clinical trial: So far, no clinical study has been reported.

References:
[1] Wu YD,Lou YJ.  Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostate cancer PC-3 cells. Pharmazie.2007 May;62(5):392-5.
[2] CHEN Shi-ping, HE Jia, WANG Qi-jing, WANG Jian-dong, YE Yan-li, XIAN Li-jian.  Effect of Brassinolide on Levels of Blood Glucose in Alloxan—induced Diabetes Rats. Acta Metallurgica Sinica, 2009,V26(03): 21-23

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt480.68
Cas No.72962-43-7
FormulaC28H48O6
Synonyms24-Epibrassinolide,Brassin lactone
Solubility≥48.1 mg/mL in DMSO with gentle warming, ≥52.3 mg/mL in EtOH with ultrasonic and warming, <2.25 mg/mL in H2O
Chemical Name(3aS,5S,6R,7aR,7bS,9aS,10R,12aS,12bS)-10-((2S,3R,4R,5S)-3,4-dihydroxy-5,6-dimethylheptan-2-yl)-5,6-dihydroxy-7a,9a-dimethyltetradecahydro-1H-benzo[c]indeno[5,4-e]oxepin-3(12bH)-one
SDFDownload SDF
Canonical SMILESCC(C)[C@H](C)[C@@H](O)[C@H](O)[C@@H](C)[C@@]1([H])CC[C@@]2([H])[C@]3([H])COC([C@@]4([H])C[C@H](O)[C@H](O)C[C@]4(C)[C@@]3([H])CC[C@@]21C)=O
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

细胞实验 [1]:

细胞系

人前列腺癌PC-3细胞

溶解方法

该化合物在DMSO中的溶解度大于24.1 mg/mL。若获取更高浓度的溶液,可在37℃下孵育10分钟,随后在超声波浴中摇匀。-20℃以下可储存数月。

反应条件

10, 20和40 μM;12, 24和36 h

应用

在PC-3细胞中,Brassinolide以时间和浓度依赖性方式诱导细胞毒性。Brassinolide(10、20和40 μM,12小时)以浓度依赖性方式增加细胞凋亡率和G2/M期细胞周期的积累。Brassinolide(20 μM,24小时)处理的PC-3细胞表现出特征性的凋亡改变:细胞数收缩、细胞表面微绒毛减少、细胞质空泡及染色质凝结。Brassinolide(20 μM)处理6、12和18小时的PC-3细胞中,caspases-3的活性以时间依赖性方式增加。

动物实验 [2]:

动物模型

糖尿病大鼠

给药剂量

口服,200、100和50 mg/kg,每天一次,连续7天

应用

口服给药Brassinolide将血糖水平从19.71-24.10 mmol/L降至9.89 mmol/L-12.70 mmol/L。使用不同剂量的Brassinolide治疗后,血糖水平差异显著。甚至在较低剂量下,Brassinolide也可以降低血糖水平,没有毒性作用。

注意事项

由于实验环境的不同,实际溶解度可能与理论值略有不同,请测试室内所有化合物的溶解度。

References:

[1]. Wu Y D, Lou Y J. Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostate cancer PC-3 cells[J]. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 2007, 62(5): 392-395.

[2]. CHEN S, HE J, WANG Q, et al. Effect of Brassinolide on Levels of Blood Glucose in Alloxan—induced Diabetes Rats [J][J]. Laboratory Animal Science, 2009, 3: 007.

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