BMH-21
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
BMH-21是平面杂环小分子DNA嵌入剂,抑制聚合酶I活性的IC50值为0.06 M[1].
与DNA相互作用是多类抗癌药的公认特性,通过三种典型的结合形式与双螺旋DNA发生相互作用,即DNA插入\凹槽结合和共价相互作用.[1]
BMH-21可插入dsDNA,并对富含GC的DNA序列有偏爱性.BMH-21对人类癌细胞系具有广泛的细胞毒性,作用方式不依赖p53,而p53被广泛认为是多种细胞毒性药物的介质.BMH-21是一种新的抑制RNA聚合酶I(Pol I)转录的制剂,可结合核糖体(r)DNA,阻断Pol I并降解Pol I大催化亚基RPA194.BMH-21可引起核仁结构的解体.[1] BMH-21可诱导人类前列腺组织上皮隔的p53表达,表明其组织渗透性.[2]
参考文献：
[1]. Colis L, Peltonen K, Sirajuddin P et al. DNA intercalator BMH-21 inhibits RNA polymerase I independent of DNA damage response. Oncotarget. 2014 Jun 30;5(12):4361-9.
[2]. Peltonen K, Colis L, Liu H et al. Identification of novel p53 pathway activating small-molecule compounds reveals unexpected similarities with known therapeutic agents. PLoS One. 2010 Sep 27;5(9):e12996.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 360.41 |
| Cas No. | 896705-16-1 |
| Formula | C21H20N4O2 |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥7.4 mg/mL in DMSO |
| Chemical Name | N-(2-(dimethylamino)ethyl)-12-oxo-12H-benzo[g]pyrido[2,1-b]quinazoline-4-carboxamide |
| SDF | Download SDF |
| Canonical SMILES | O=C1C2=C(C=C(C=CC=C3)C3=C2)N=C4C(C(NCCN(C)C)=O)=CC=CN41 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
