BIX 02188
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
BIX02188是MEK5的选择性抑制剂,IC50值为4.3 nM。
BIX02188属于二氢吲哚酮激酶抑制剂系列,它选择性抑制MEK5的催化活性,但不作用于其他密切相关的激酶,如MEK1、MEK2、ERK2和JNK2。BIX02188也抑制ERK5,IC50值为810 nM。在HeLa细胞中,使用BIX02188处理,抑制ERK5的磷酸化,但对ERK1/2没有影响。除此之外,BIX02188阻止HeLa和HEK293细胞中下游底物MEF2C的转录。对79种激酶进行测试,BIX02188对CSF1R、KIT和LCK激酶表现出一定程度的抑制,IC50值分别为280 nM、 550 nM和390 nM。
参考文献:
1. Tatake R J, O’Neill M M, Kennedy C A, et al. Identification of pharmacological inhibitors of the MEK5/ERK5 pathway. Biochemical and biophysical research communications, 2008, 377(1): 120-125.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 412.48 |
Cas No. | 1094614-84-2 |
Formula | C25H24N4O2 |
Solubility | insoluble in H2O; insoluble in EtOH; ≥10.3 mg/mL in DMSO |
Chemical Name | (3E)-3-[[3-[(dimethylamino)methyl]anilino]-phenylmethylidene]-2-oxo-1H-indole-6-carboxamide |
SDF | Download SDF |
Canonical SMILES | CN(C)CC1=CC(=CC=C1)NC(=C2C3=C(C=C(C=C3)C(=O)N)NC2=O)C4=CC=CC=C4 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Description | BIX02188是MEK5的选择性抑制剂,IC50值为4.3 nM。 | |||||
靶点 | MEK5 | ERK5 | TGFβR1 | |||
IC50 | 4.3 nM | 810 nM | 1.8 μM |
质量控制和MSDS
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