Bicyclomycin Benzoate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Bicyclomycin benzoate is a novel antibiotic produced by S. sapporonensi [1]. Bicyclomycin shows an inhibitory effect on Gram-negative bacteria, such as E. coli, Klebsiella, Shigella, Salmonella, Citrobacter, E. cloacae, and the pathogenic group of Neisseria [2]. Bicyclomycin benzoate is inactive against Proteus, P. aeruginosa, and Gram-positive bacteria [1,2].
In vitro: The MIG of bicyclomycin for E.coli NIHJ JC-2 was 25-50 μg/ml. Bicyclomycin (25-50 μg/ml) completely inhibited formation of visible colonies or turbidity of this organism on agar plates, in nutrient broth, and in heart infusion broth [2]. Bicyclomycin inhibited ATPase activity in the presence of poly(dC) and ribo(C)10. The approximate IC50 value for inhibition of transcription termination at Rho-dependent sites was 5 μM. The inhibitory effect of bicyclomycin on Rho-dependent transcripts was accompanied by the appearance of a new set of transcripts. In the presence of poly(dC), bicyclomycin reversibly inhibited the ribo(C)10-stimulated ATPase activity. The extrapolated Ki for bicyclomycin was 2.8 μM without ribo(C)10, which was increased to 26 μM in the presence of ribo(C)10 [3].
In vivo: Bicyclomycin showed therapeutic activity for infections with several strains of E. coli which were resistant to the control antibiotics. The ED50 of bicyclomycin for infection with GP-resistant E.coli 312 was 3.05 (1.47-7.66) mg/mouse [2].
References:
[1] Miyoshi, T. ,Miyairi, N.,Aoki, H., et al. Bicyclomycin, a new antibiotic. I. Taxonomy, isolation and characterization. J.Antibiot.(Tokyo) 25(10), 569-575(1972).
[2] Nishida, M. ,Mine, Y.,Matsubara, T., et al. Bicyclomycin, a new antibiotic. III. In vitro and in vivo antimicrobial activity. J.Antibiot.(Tokyo) 25(10), 582-593(1972).
[3] Magyar A, Zhang X, Kohn H, et al. The antibiotic bicyclomycin affects the secondary RNA binding site of Escherichia coli transcription termination factor Rho[J]. Journal of Biological Chemistry, 1996, 271(41): 25369-25374.
Physical Appearance | A white solid |
Storage | Store at -20°C |
M.Wt | 406.4 |
Cas No. | 37134-40-0 |
Formula | C19H22N2O8 |
Synonyms | FR2054 |
Solubility | Soluble in DMSO |
Chemical Name | 3'-benzoate bicyclomycin |
SDF | Download SDF |
Canonical SMILES | O=C1[C@@](O)(NC2=O)C(CCO[C@]2([C@@H](O)[C@@](C)(O)COC(C3=CC=CC=C3)=O)N1)=C |
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