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BAFF, human recombinant protein

 
Catalog No.
P1044
BAFF,B细胞激活因子,在大肠杆菌中表达。
组合的产品项目
规格价格库存 数量
5ug
¥ 886.00
Ship with 5-10 days
100ug
¥ 12,686.00
Ship with 5-10 days
500ug
¥ 28,463.00
Ship with 5-10 days

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A

背景

B-cell activating factor (BAFF), also known as Blys, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation. BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56 % a.a. sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. 

Reference:

1. Craxton A, Draves KE, Gruppi A, et al. 2005. J Exp Med, 202: 1363-74

2. Kalled SL. 2005. Immunol Rev, 204: 43-54

3. Kuo SH, Yeh PY, Chen LT, et al. 2008. Blood, 112: 2927-34

4. Rowland SL, Leahy KF, Halverson R, et al. 2010. J Immunol, 185: 4570-81

5. Liu ZandDavidson A. 2011. Exp Cell Res, 317: 1270-7.

Description

Gene ID

10673

Accession #

Q9Y275

Alternate Names

TNFSF13B, TNFSF20, TALL-1, BLys, THANK, B cell Activating Factor belonging to the TNF family

Source

Escherichia coli.

M.Wt

Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.

AA Sequence

MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Formulation

 

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a mouse splenocyte survival assay is 0.5-2 µg/ml.

Shipping Condition

Gel pack.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans.

 

质量控制

质量控制和DataSheet

批次:
    纯度 > 95 % by SDS-PAGE and HPLC analyses.
    Endotoxin: Less than 1 EU/μg of rHuBAFF as determined by LAL method.
  • Datasheet

相关生物数据

FGF-21