AST-1306
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AST 1306是EGFR和HER2的新型抑制剂,IC50分别为0.5 nM和3 nM。
表皮生长因子受体(EGFR)是一种细胞表面受体酪氨酸激酶。受体活化导致二聚化和酪氨酸自磷酸化。它诱导一系列下游细胞应答级联反应,如基因表达修饰、细胞增殖和细胞骨架重排等。HER2是表皮生长因子的一员,与乳腺癌和卵巢癌有关。
在无细胞实验中,AST1306选择性阻断EGFR和HER2激酶的活性。在完整的细胞和无细胞实验中,AST1306可以抑制EGFR突变T790M/L858R的酪氨酸激酶活性。此外,AST1306减弱EGFR和HER2的磷酸化和下游底物[1]。
在ErbB2过表达的异种移植小鼠模型和FVB-2/Nneu转基因小鼠模型中,AST1306有效抑制肿瘤生长。在SK-OV-3异种移植模型中,AST1306对EGFR和HER2产生了快速和长效的抑制,大于等于24h[1]。
参考文献:
1. Xie H, Lin L, Tong L et al. AST1306, a novel irreversible inhibitor of the epidermal growth factor receptor 1 and 2, exhibits antitumor activity both in vitro and in vivo. PLoS One. 2011;6(7):e21487.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 448.88 |
Cas No. | 897383-62-9 |
Formula | C24H18ClFN4O2 |
Synonyms | AST1306; AST 1306 |
Solubility | ≥22.45 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
Chemical Name | N-[4-[3-chloro-4-[(3-fluorophenyl)methoxy]anilino]quinazolin-6-yl]prop-2-enamide |
SDF | Download SDF |
Canonical SMILES | C=CC(=O)NC1=CC2=C(C=C1)N=CN=C2NC3=CC(=C(C=C3)OCC4=CC(=CC=C4)F)Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |