AS-604850
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AS-604850是一种选择性的和ATP竞争性的PI3Kγ抑制剂(IC50= 2.5 μM),比对PI3Kδ和PI3Kβ的选择性高30多倍,比对PI3Kα的选择性高18倍.[1]
PI3K(磷脂酰肌醇-4,5-二磷酸-3-激酶)是一类参与细胞功能如细胞生长\增殖\分化\运动\存活和胞内运输的酶,从而参与癌症.该物质在PI3K/Akt(PKB)/mTOR途径中扮演关键角色.
在大鼠肝细胞中,AS-604850预处理可消除50%的GCDC诱导的Akt磷酸化,并能减少GCDC/TCDC/TLC诱导的caspase-3剪切,抑制细胞凋亡.[2]在PI3KCG-/-单核细胞和THP-1(人类单核细胞系)中,AS-604850可抑制MCP-1激活的PKB磷酸化,也可以浓度依赖的方式阻断MCP-1介导的趋化作用.在RAW264小鼠巨噬细胞中,C5a介导的PKB磷酸化也可被AS-604850抑制.[2]
在体内诱导多种趋化因子的巯基乙酸诱导腹膜炎模型中,口服10 mg/kg的AS-604850可降低31%的中性粒细胞募集.[1]
参考文献:
1. Camps M, Rückle T, Ji H et al. Blockade of PI3K-gamma suppresses joint inflammation and damage in mouse models of rheumatoid arthritis. Nat Med. 2005 Sep;11(9):936-43.
2. Hohenester S, Gates A, Wimmer R et al. Phosphatidylinositol-3-kinase p110γ contributes to bile salt-induced apoptosis in primary rat hepatocytes and human hepatoma cells. J Hepatol. 2010 Nov;53(5):918-26.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 285.22 |
Cas No. | 648449-76-7 |
Formula | C11H5F2NO4S |
Solubility | Soluble in DMSO |
Chemical Name | 5-[(2,2-difluoro-1,3-benzodioxol-5-yl)methylidene]-1,3-thiazolidine-2,4-dione |
SDF | Download SDF |
Canonical SMILES | C1=CC2=C(C=C1C=C3C(=O)NC(=O)S3)OC(O2)(F)F |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |