AS 19
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
5-HT7受体在调节睡眠和昼夜节律中发挥作用.此受体通过中枢和外周机制,也在其他CNS疾病中发挥一定的作用,包括认知障碍\焦虑和偏头痛[2].
通过5-HT7受体传递信号,5-羟色胺为增强T细胞活化提供辅助信号.相比于盐处理的对照,PCPA处理的小鼠中T细胞增殖受到显著抑制.PCPA的抑制效应约为18%-49%.加入1 μM 外源性5-HT能够完全恢复T细胞增殖.类似于外源性5-HT,加入1 μM AS 19也能于48小时后完全恢复T细胞增殖能力[4].
SB-258719是选择性的5-HT7受体拮抗剂.10 mg/kg的AS-19与同剂量的SB-258719协同处理(对其本身无效应),在热板和甩尾实验中,AS-19的抗痛觉过敏效应皆被逆转.表明AS-19通过5-HT7受体发挥抗痛觉过敏效应[3].SB269970也是选择性5-HT7受体拮抗剂.一些大鼠通过第二鞘内导管用7 μl 5 mM的SB269970预处理,在所有时间节点,AS-19诱导的PMF被SB269970的预处理所废除,表明AS-19通过5-HT7受体的活化而产生PMF[5].
参考文献:
[1]. Perez-García GS and Meneses A. Effects of the potential 5-HT7 receptor agonist AS 19 in an autoshaping learning task. Behav Brain Res, 2005, 163(1):136-40.
[2]. Thomas DR and Hagan JJ. 5-HT7 Receptors. Current Drug Targets-CNS & Neurological Disorders, 2004, 3(1): 81-90.
[3]. Ulugol A, Oltulu C, Gunduz O, et al. 5-HT7 receptor activation attenuates thermal hyperalgesia in streptozocin-induced diabetic mice. Pharmacol Biochem Behav, 2012, 102(2):344-8.
[4]. León-Ponte M, Ahern GP and O'Connell PJ. Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor. Blood, 2007, 109(8):3139-46.
[5]. Hoffman MS and Mitchell GS. Spinal 5-HT7 receptor activation induces long-lasting phrenic motor facilitation. J Physiol, 2011, 589(Pt 6):1397-407.
Physical Appearance | Yellow oil |
Storage | Desiccate at -20°C |
M.Wt | 283.41 |
Cas No. | 1000578-26-6 |
Formula | C18H25N3 |
Solubility | <28.34mg/ml in ethanol; <28.34mg/ml in DMSO |
Chemical Name | (2S)-N,N-dimethyl-8-(1,3,5-trimethyl-1H-pyrazol-4-yl)-1,2,3,4-tetrahydronaphthalen-2-amine |
SDF | Download SDF |
Canonical SMILES | CC1=NN(C(C)=C1C2=CC=CC3=C2C[C@](N(C)C)([H])CC3)C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |