Apoptosis Activator 2
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Apoptosis Activator 2是一种小分子细胞凋亡激活剂,IC50值约为4 μM[1]。
Apoptosis Activator 2是一种细胞可透过的物质,可通过细胞色素c和Apaf-1依赖的方式活化caspases,诱发细胞凋亡。20 μM的Apoptosis Activator 2在0.15 μM细胞色素c存在时可提高凋亡体中Apaf-1的含量,可诱导Apaf-1的寡聚化。通过诱发细胞凋亡,Apoptosis Activator 2显著诱导caspase-3激活、PARP剪切和DNA碎片化。此外,这种细胞凋亡诱导只在肿瘤细胞系(如乳腺、肺、结肠和表皮癌细胞系)中发生。正常细胞系可抗Apoptosis Activator 2[1,2]。
参考文献:
[1] Nguyen JT, Wells JA. Direct activation of the apoptosis machinery as a mechanism to target cancer cells. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7533-8.
[2] Nguyen TV, Jayaraman A, Quaglino A, Pike CJ. Androgens selectively protect against apoptosis in hippocampal neurones. J Neuroendocrinol. 2010 Sep;22(9):1013-22.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 306.1 |
Cas No. | 79183-19-0 |
Formula | C15H9Cl2NO2 |
Solubility | Soluble in DMSO |
Chemical Name | 1-[(3,4-dichlorophenyl)methyl]indole-2,3-dione |
SDF | Download SDF |
Canonical SMILES | C1=CC=C2C(=C1)C(=O)C(=O)N2CC3=CC(=C(C=C3)Cl)Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Description | Apoptosis Activator 2是一种细胞可透过的吲哚二酮caspase激活剂,在白血病细胞中的IC50值为4-9 μM。 | |||||
靶点 | caspase | |||||
IC50 |