ApexPrep DNA Plasmid Miniprep Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
本试剂盒采用碱裂解法裂解细胞，再通过离心吸附柱在高盐状态下特异性地结合溶液中的DNA，洗去杂质，可高效快速提取多至30 μg的质粒DNA。本试剂盒采用独特的缓冲液配方，最大限度去除蛋白杂质及其它有机化合物，每次可处理1-5 mL菌液。质粒提取得率和质量与宿主菌的种类和培养条件，细胞的裂解，质粒拷贝数，质粒的稳定性，抗生素等因素有关。
使用本试剂盒提取的质粒DNA可适用于各种常规操作，包括酶切、PCR、测序、连接、 转化、文库筛选、体外翻译、转染一些常规的传代细胞等。
- 1. Xiang Song Jian Gong, et al. "Plasma-based early screening and monitoring of EGFR mutations in NSCLC patients by a 3-color digital PCR assay." Br J Cancer. 2020 Oct;123(9):1437-1444. PMID:32782293
- 2. Wang EX, Ding MZ, et al. "Reorganization of a synthetic microbial consortium for one-step vitamin C fermentation." Microb Cell Fact. 2016 Jan 25;15:21. PMID:26809519
| Storage | 室温保存，一年有效 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Components | 50 tests | 300 tests |
| Buffer BL | 30 mL | 160 mL |
| Buffer A1 | 20 mL | 100 mL |
| Buffer A2 | 20 mL | 100 mL |
| Buffer A3 | 30 mL | 120 mL |
| Buffer AP | 12 mL | 50 mL |
| Buffer AE | 15 mL | 30 mL |
| RNase A | 200 μL (10 mg/mL) | 1 mL (10 mg/mL) |
| 吸附柱 | 50个 | 300个 |
| 收集管 | 50个 | 300个 |
室温保存，一年有效。加入RNase A的缓冲液A1于2-8°C保存。 | ||
For purification of up to 20 μg molecular biology grade plasmid DNA
- Ready-to-use plasmid DNA in minutes
- Reproducible yields of molecular biology grade plasmid DNA
- Single protocol for high- and low-copy vectors
The ApexPrep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.
