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EZLink Sulfo-NHS-LC-Biotin Kit

现货
Catalog No.
K1001
Amine-reactive biotinyltation kit, water-soluble
组合的产品项目
规格价格库存 数量
10 reactions
¥ 2,160.00
Ship with 10-15 days

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Description

The EZLink Sulfo-NHS-LC-Biotin Kit contains reagents sufficient for 10 biotin labeling reactions. It is a water-soluble biotin reagent for labeling protein, antibody or other molecules with amidogen (NH2-). The biotin in the kit is sufficient and has been activated for direct use. Each kit includes 10 Filtration tubes for desaltination of antibody labeling without the need for dialysis. The whole procedure is simple and quick.

Components and Storage

Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
Streptavidin10 mgEZ-Link Sulfo-NHS-LC-Biotin25 mg
HABA Solution1 mLPBS Pack (makes 500 mL)1 pack
Sephadex G-25 in PD-10 Desalting Columns8.3 mL, 10 columns
Store biotin and streptavidin reagents at -20°C. Store remaining kit components at 4°C.

Specification

Label or Dye:BiotinProduct Size:10 reactions
Labeling Scale:1-10 mgLabeling Target:Proteins (General), Antibodies (General)

Specialty

• Protein labeling—biotin antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Antibody labeling—biotin antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling—biotin only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Medium length—spacer arm (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass

Sulfo-NHS-LC-Biotin is one of three very similar EZ-Link Reagents that are water-soluble, non-cleavable, and enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three Sulfo-NHS-ester reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Sulfo-NHS-LC-Biotin is offered in several package sizes and as complete protein labeling kits.