Anisomycin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Anisomycin是JNK特异性的激动剂,作用浓度为25 ng / ml[1]。
JNK是c-Jun N端激酶的缩写,作为促凋亡激酶,在许多细胞进程例如细胞周期、增殖、凋亡和细胞应激中起重要作用。研究表明,JNK在UV诱导的细胞凋亡中发挥重要作用,JNK途径的激活可以加强TNF-α介导的细胞凋亡,因此,JNK被认为是临床的重要靶标[2,3]。
Anisomycin是一种强效的JNK激动剂。在细胞系DU145(对Fas介导的凋亡具有较高抗性)中,250 ng/ml anisomysin和Fas (200 ng/ml)共同作用通过激活JNK,诱导DU145细胞凋亡[4]。在HL-60细胞中,使用anisomysin活化JNK途径可以诱导细胞凋亡[5]。在原代鼠胚胎成纤维细胞中,anisomycin通过激活JNK的表达诱导细胞凋亡[6]。
参考文献:
[1].Jiang, J., et al., Spermassociated antigen 9 promotes astrocytoma cell invasion through the upregulation of podocalyxin. Mol Med Rep, 2014. 10(1): p. 417-22.
[2].Lin, A., Activation of the JNK signaling pathway: breaking the brake on apoptosis. Bioessays, 2003. 25(1): p. 17-24.
[3].Liu, J. and A. Lin, Role of JNK activation in apoptosis: a double-edged sword. Cell Res, 2005. 15(1): p. 36-42.
[4].Curtin, J.F. and T.G. Cotter, Anisomycin activates JNK and sensitises DU 145 prostate carcinoma cells to Fas mediated apoptosis. Br J Cancer, 2002. 87(10): p. 1188-94.
[5].Stadheim, T.A. and G.L. Kucera, c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) is required for mitoxantrone- and anisomycin-induced apoptosis in HL-60 cells. Leuk Res, 2002. 26(1): p. 55-65.
[6].Tournier, C., et al., Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway. Science, 2000. 288(5467): p. 870-4.
- 1. De-qing Yang, Qiu-nan Zuo, et al. "Mitochondrial-Targeting Antioxidant SS-31 Suppresses Airway Inflammation and Oxidative Stress Induced by Cigarette Smoke." Oxid Med Cell Longev. 2021 Jun 15;2021:6644238. PMID:34221235
- 2. Li Wan , Szymon Juszkiewicz, et al. "Translation stress and collided ribosomes are co-activators of cGAS." Mol Cell. 2021 Jul 1;81(13):2808-2822.e10. PMID:34111399
- 3. Niu J, Li Z, et al. "Overexpressed microRNA-136 works as a cancer suppressor in gallbladder cancer through suppression of JNK signaling pathway via inhibition of MAP2K4." Am J Physiol Gastrointest Liver Physiol. 2019 Aug 1. PMID:31369289
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 265.31 |
Cas No. | 22862-76-6 |
Formula | C14H19NO4 |
Solubility | ≥26.5 mg/mL in DMSO; insoluble in H2O; ≥30.55 mg/mL in EtOH |
Chemical Name | (2R,3S,4S)-4-hydroxy-2-(4-methoxybenzyl)pyrrolidin-3-yl acetate |
SDF | Download SDF |
Canonical SMILES | O[C@@H]1[C@H]([C@@H](CC(C=C2)=CC=C2OC)NC1)OC(C)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Cell experiment:[1] | |
Cell lines |
Hormone refractory cell line DU 145 |
Reaction Conditions |
250 ng/ml anisomycin for 8 h incubation |
Applications |
In DU 145 cells, anisomycin activated JNK, and acted in synergy with anti-Fas IgM to induce apoptosis. Furthermore, anisomycin was found to activate JNK activation over a prolonged period, whilst anti-Fas IgM was unable to induce transient (1 h) or prolonged (8 h) JNK activation in DU 145 cells. |
Animal experiment:[2] | |
Animal models |
Ehrlich ascites carcinoma (EAC)-bearing mice |
Dosage form |
5 mg/kg Injected peritumorally every other day for 7 times |
Applications |
Peritumoral administration of anisomycin (5 mg/kg) significantly suppressed EAC growth, resulting in the survival of approximately 60% of the mice 90 days after EAC inoculation. Enhancement of infiltrating lymphocytes was noted in the tumor tissue, which was dramatically superior to adriamycin. |
Note |
The technical data provided above is for reference only. |
References: 1. Curtin JF, Cotter TG. Anisomycin activates JNK and sensitises DU 145 prostate carcinoma cells to Fas mediated apoptosis. British Journal of Cancer, 2002, 87(10): 1188-1194. 2. You P, Xing F, Huo J, et al. In vitro and in vivo evaluation of anisomycin against Ehrlich ascites carcinoma. Oncology Reports, 2013, 29(6): 2227-2236. |
质量控制和MSDS
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