Anguizole
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Anguizole是NS4B的选择性抑制剂，IC50为310 nM [1]。
NS4B（非结构蛋白4B）是C型肝炎病毒中的关键蛋白，在HCV复制复合体形成中发挥重要作用。据悉，NS4B通过诱导特异性膜重排和选定膜系统，在 HCV复制中发挥重要作用。NS4B-AH2包含氨基酸42至66，是NS4B寡聚化的重要调节因子[2]。
Anguizole是NS4B的强效抑制剂，靶向结合NS4B（NS4B-AH2）的第二螺旋。在HCV病毒中，anguizole显著扰乱NS4B-AH2介导的NS4B的生物学功能，而NS4B在HCV的生命周期中发挥重要作用。在转染表达融合GFP-NS4B质粒的Huh7.5细胞中，5 μM anguizole处理48 h后改变了NS4B MAF的分布，抑制HCV的生命周期[1]。
参考文献：?
[1].?Bryson, P.D., et al., A small molecule inhibits HCV replication and alters NS4B's subcellular distribution. Antiviral Res, 2010. 87(1): p. 1-8.
[2].?Gouttenoire, J., et al., Aminoterminal amphipathic alpha-helix AH1 of hepatitis C virus nonstructural protein 4B possesses a dual role in RNA replication and virus production. PLoS Pathog, 2014. 10(10): p. e1004501.
[3].?Choi, M., et al., A hepatitis C virus NS4B inhibitor suppresses viral genome replication by disrupting NS4B's dimerization/multimerization as well as its interaction with NS5A. Virus Genes, 2013. 47(3): p. 395-407.
| Storage | Store at -20°C |
| M.Wt | 408.81 |
| Cas No. | 442666-98-0 |
| Formula | C17H11ClF2N4O2S |
| Synonyms | 7-[chloro(difluoro)methyl]-5-(furan-2-yl)-N-(thiophen-2-ylmethyl)pyrazolo[1,5-a]pyrimidine-2-carboxamide |
| Solubility | Soluble in DMSO |
| Chemical Name | 7-[chloro(difluoro)methyl]-5-(furan-2-yl)-N-(thiophen-2-ylmethyl)pyrazolo[1,5-a]pyrimidine-2-carboxamide |
| SDF | Download SDF |
| Canonical SMILES | C1=COC(=C1)C2=NC3=CC(=NN3C(=C2)C(F)(F)Cl)C(=O)NCC4=CC=CS4 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 描述 | Anguizole是HCV复制的小分子抑制剂，可以改变NS4B的亚细胞分布。 | |||||
| 靶点 | HCV | |||||
| IC50 | ||||||
化学结构
