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Angiotensin II

现货
Catalog No.
A1042
有效的血管加压剂,强烈刺激肾上腺皮质产生和释放醛固酮。
组合的产品项目
规格价格库存 数量
5mg
¥ 300.00
现货
10mg
¥ 500.00
现货
25mg
¥ 700.00
现货
250mg
¥ 5,000.00
现货

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Background

Angiotensin II (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe | His-Leu) is an octapeptide that is converted from Angiotensin I (AI) through removal of two C-terminal residues by the enzyme angiotensin-converting enzyme (ACE), primarily through ACE within the lung (but also present in endothelial and kidney epithelial cells).

Angiotensin II has been shown to play important roles in mediating hypertension, heart failure, cardiac remodeling, diabetes, and the proliferative and inflammatory responses to arterial injury [1].

Angiotensin II exerts a wide range of effects on the cardiovascular system. It is a potent vasopressor and a powerful stimulus for production and release of aldosterone from the adrenal cortex. It acts via specific receptors in the adrenal glands to stimulate the secretion of aldosterone, which stimulates salt and water reabsorption by the kidneys. Angiotensin increases blood pressure by stimulating the Gq protein in vascular smooth muscle cells (which in turn activates contraction by an IP3-dependent mechanism). It was demonstrated that Ang II activated phospholipase C, resulting in the production of inositol trisphosphate (IP3) and diacylglycerol, which in turn were responsible for the mobilization of [Ca2+]i and the activation of PKC, respectively. Additional studies in SMC cultures examined the properties of Ang II as a growth agonist, in particular its role in promoting cellular hypertrophy, characterized by increases in protein synthesis, cell size, and polyploidy. [2][3]

A1042_1 

 Fig. 2: Formula of Angiotensin II

A1042_2 

Fig. 2: Structure of Angiotensin II

 

Ref:

1. Ruiz-Ortega M, Lorenzo O, Ruperez M, Esteban V, Suzuki Y, Mezzano S, Plaza JJ, Egido J. Role of the renin-angiotensin system in vascular diseases: expanding the field. Hypertension. 2001; 38: 1382–1387.

2. Geisterfer AA, Peach MJ, Owens GK. Angiotensin II induces hypertrophy, not hyperplasia, of cultured rat aortic smooth muscle cells. Circ Res. 1988; 62: 749–756.

3. Berk BC, Vekshtein V, Gordon HM, Tsuda T. Angiotensin II-stimulated protein synthesis in cultured vascular smooth muscle cells. Hypertension. 1989; 13: 305–314.

Chemical Properties

StorageDesiccate at -20°C
M.Wt1046.2
Cas No.4474-91-3
FormulaC50H71N13O12
SynonymsAsp-Arg-Val-Tyr-Ile-His-Pro-Phe
Solubility≥100.2mg/mL in H2O, <2.09mg/mL in DMSO
Chemical NameAngiotensin II
SDFDownload SDF
Canonical SMILESCCC(C)C(C(=O)NC(CC1=CN=CN1)C(=O)N2CCCC2C(=O)NC(CC3=CC=CC=C3)C(=O)O)NC(=O)C(CC4=CC=C(C=C4)O)NC(=O)C(C(C)C)NC(=O)C(CCCN=C(N)N)NC(=O)C(CC(=O)O)N.CC(=O)O
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

细胞实验[1] :

细胞系

血管平滑肌细胞

溶解方法

Angiotensin II在无菌水中的溶解度大于10 mM,原液需分装并保存在-80°C,可保存数月。

反应条件

100 nM, 4 小时

实验结果

使用血管紧张素II处理可以大量增加NADH和NADPH氧化酶的活性,血管紧张素II对于酶活性的初始速率和峰值响应均有影响。处理后1小时之内,氧化酶活性的增加不明显,1小时之后,氧化酶活性持续增加至少6小时。

动物实验 [2] :

动物模型

C57BL/6J (apoE–/–) 小鼠

剂量

药物通过置于颈后部皮下的微泵输送,500或1000ng/min/kg持续28天。

实验结果

Ang II输注可以促进腹主动脉瘤的发生。来自Ang II-输注的小鼠的腹主动脉区域的尺寸显著增加。包含该区域的组织对典型的除去外膜组织的解剖过程具有抗性。在使用500和1,000ng/min/kg的Ang II输注的实验组中,分别有20%和33%的小鼠出现球状主动脉腹部形状。

注意事项

请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的,属于正常现象。

References:

[1] Griendling K K, Minieri C A, Ollerenshaw J D, et al. Angiotensin II stimulates NADH and NADPH oxidase activity in cultured vascular smooth muscle cells. Circulation research, 1994, 74(6): 1141-1148.

[2] Daugherty A, Manning M W, Cassis L A. Angiotensin II promotes atherosclerotic lesions and aneurysms in apolipoprotein E–deficient mice. Journal of Clinical Investigation, 2000, 105(11): 1605-1612.

质量控制

化学结构

Angiotensin II