Andrographolide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Andrographolide对α-葡萄糖苷酶和α-淀粉酶的IC50值分别为11.0 ± 0.28 μg/ml和11.3 ± 0.29 μg/ml。
Andrographolide是一种不可逆的NF-κB拮抗剂。基于NF-κB对促炎症基因表达的作用，包括趋化因子、细胞因子以及粘附分子，核因子NF-κB通路被认为是一种典型的促炎症信号通路。
体外：Andrographolide浓度依赖性地抑制诱导型一氧化氮合酶表达。在Andrographolide治疗组中，观察到Akt、c-Jun氨基末端激酶以及p65磷酸化的减少。Andrographolide减少Bcl-2/NF-κb表达，并剂量依赖性地增加裂解Caspase3/Bax蛋白表达[1]。
体内：在一项肝脏保护实验中，大鼠灌胃给予Andrographolide，连续5天。结果表明，在心脏、肝脏和肾脏中，Andrographolide能上调谷氨酸半胱氨酸连接酶的催化与修饰亚基、血红素加氧酶-1、超氧化物歧化酶-1、谷胱甘肽S-转移酶的蛋白以及mRNA表达[2]。
临床试验：N/A。
参考文献：
[1] Wang ZM, Kang YH, Yang X, Wang JF, Zhang Q, Yang BX, Zhao KL, Xu LP, Yang LP, Ma JX, Huang GH, Cai J, Sun XC. Andrographolide radiosensitizes human esophageal cancer cell line ECA109 to radiation in vitro. Dis Esophagus. 2016 Jan;29(1):54-61.
[2] Chen HW, Huang CS, Li CC, Lin AH, Huang YJ, Wang TS, Yao HT, Lii CK. Bioavailability of andrographolide and protection against carbon tetrachloride-induced oxidative damage in rats. Toxicol Appl Pharmacol. 2014 Oct 1;280(1):1-9.
- 1. Siyi Che, Na Zhou, et al. "Andrographolide exerts anti-respiratory syncytial virus activity by up-regulating heme oxygenase-1 independent of interferon responses in human airway epithelial cells." Research Square. August 19th, 2022.
- 2. Siyi Che, Xiaohong Xie, et al. "Andrographolide attenuates RSV-induced inflammation by suppressing apoptosis and promoting pyroptosis after respiratory syncytial virus infection in vitro." Research Square. March 15th, 2022.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 350.44 |
| Cas No. | 5508-58-7 |
| Formula | C20H30O5 |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥16 mg/mL in DMSO |
| Chemical Name | (3E,4S)-3-[2-[(1R,4aS,5R,6R,8aS)-6-hydroxy-5-(hydroxymethyl)-5,8a-dimethyl-2-methylidene-3,4,4a,6,7,8-hexahydro-1H-naphthalen-1-yl]ethylidene]-4-hydroxyoxolan-2-one |
| SDF | Download SDF |
| Canonical SMILES | C=C1CC[C@]2([H])[C@@](CO)(C)[C@H](O)CC[C@@]2(C)[C@@H]1C/C=C(C(OC3)=O)\[C@@H]3O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
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