Aminoallyl-dUTP
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Aminoallyl-dUTP用于通过聚合酶链反应（PCR）（Taq聚合酶）、切口平移（DNAse I / DNA 聚合酶I）、引物延伸（Klenow exo-）、3端标记（末端脱氧核苷酸转移酶，TdT）和逆转录（MMLV逆转录酶）进行DNA / cDNA的间接稳定标记。Aminoallyl-dUTP作为其天然配对物dTTP的替代物，被插入DNA / cDNA中。产生的胺官能化的DNA/cDNA可以通过经典的胺/ NHS酯反应引入生物素基团（通过生物素的NHS酯），用于后续的纯化任务，或通过荧光染料的NHS酯引入荧光基团，用于后续的显微成像和斑点印迹（cDNA探针）[1] [2]。
Aminoallyl-dUTP也可以通过PCR被插入探针中，并作为随后标记探针的反应位点。荧光标记探针可用于原位杂交[2]。
参考文献：
[1] Dirsch et al. (2007) Probe production for in situ hybridization by PCR and subsequent covalent labeling with fluorescent dyes. Appl. Immunohistochem. Mol. Morphol. 3:332.
[2] Cox et al. (2004) Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. BioTechniques 36:114.
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 523.22(free acid) |
| Cas No. | 116840-18-7 |
| Formula | C12H20N3O14P3(free acid) |
| Synonyms | 5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate,aa-dutp ,aa dutp,5-AA-dUTP,5-Aminoallyl-2'-deoxyuridine-5'-Triphosphate |
| Solubility | Soluble in sterile water |
| Chemical Name | ((2R,3S,5R)-5-(5-((E)-3-aminoprop-1-en-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate |
| SDF | Download SDF |
| Canonical SMILES | O=C(N1)N([C@@H]2O[C@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)[C@@H](O)C2)C=C(/C=C/CN)C1=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
