AM 114
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AM 114是硼酸查尔的衍生物,一种有效的小分子蛋白酶体抑制剂,抑制20S蛋白酶体的糜蛋白酶样活性,IC50值接近1 μM,可在不明显破坏p53和MDM2蛋白相互作用的情况下导致全细胞中泛素化p53和其他细胞内蛋白的大量积累。AM 114也具有抗癌活性,可有效抑制人结肠癌HCT116细胞的生长,在MTT和集落形成试验中的IC50值分别为1.5 μM和0.6μM [1]。
参考文献:
[1] Achanta G, Modzelewska A, Feng L, Khan SR, Huang P. A boronic-chalcone derivative exhibits potent anticancer activity through inhibition of the proteasome. Mol Pharmacol. 2006 Jul;70(1):426-33. Epub 2006 Apr 24.
- 1.Gunderwala A, Porter J. "A luminescence assay for natural product inhibitors of the Mycobacterium tuberculosis proteasome." Phytochem Anal. 2016 Mar;27(2):126-32. PMID:26778282
- 2.Chen, Wei, et al. "Competitive Expression of Endogenous Wheat CENH3 May Lead to Suppression of Alien ZmCENH3 in Transgenic Wheat× Maize Hybrids." Journal of Genetics and Genomics (2015).
Physical Appearance | A solid |
Storage | Store at 4°C |
M.Wt | 377.01 |
Cas No. | 856849-35-9 |
Formula | C20H21B2NO5 |
Solubility | insoluble in H2O; ≥16.65 mg/mL in DMSO; ≥4.11 mg/mL in EtOH with ultrasonic |
Chemical Name | [4-[(Z)-[(5Z)-5-[(4-boronophenyl)methylidene]-1-methyl-4-oxopiperidin-3-ylidene]methyl]phenyl]boronic acid |
SDF | Download SDF |
Canonical SMILES | B(C1=CC=C(C=C1)C=C2CN(CC(=CC3=CC=C(C=C3)B(O)O)C2=O)C)(O)O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Targets | Proteasome | |||||
IC50 | 1 μM |
质量控制和MSDS
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