AG 556
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AG 556是一种选择性的EGFR抑制剂,IC50值为1.1 μM.
表皮生长因子受体(EGFR)是表皮生长因子的细胞表面受体,在肿瘤侵袭和癌细胞增殖中起重要作用.
AG 556是一种选择性的EGFR抑制剂.在表达KIR2.3或Kir2.1的HEK 293细胞中,AG 556(10 μM)可逆地降低KIR2.3或Kir2.1电流.另外,AG 556抑制KIR2.3或Kir2.1通道的酪氨酸磷酸化.EGF (100 ng/ml)增加KIR2.3电流,而这种作用被AG556抑制[1] [2].AG556将85％的细胞阻滞在G1 / S期并通过磷酸化Cdk2上酪氨酸15,抑制Cdk2活化[3].
在感染大肠杆菌0111: B4的犬模型中,AG 556增加存活时间.AG 556增加平均动脉压\氧气输送\心输出量\肺泡动脉氧梯度和左室射血分数,这表明AG 556提高存活率.另外,AG 556显著降低血清TNF的水平,这表明AG 556抑制细胞信号传导途径和细胞因子诱导的多器官衰竭[4].
参考文献：
[1]. Zhang DY, Zhang YH, Sun HY, et al. Epidermal growth factor receptor tyrosine kinase regulates the human inward rectifier potassium K(IR)2.3 channel, stably expressed in HEK 293 cells. Br J Pharmacol, 2011 Nov, 164(5): 1469-1478 [1].
[2]. Zhang DY, Wu W, Deng XL, et al. Genistein and tyrphostin AG556 inhibit inwardly-rectifying Kir2.1 channels expressed in HEK 293 cells via protein tyrosine kinase inhibition. Biochim Biophys Acta, 2011, 1808(8): 1993-1999.
[3]. Kleinberger-Doron N, Shelah N, Capone R, et al. Inhibition of Cdk2 activation by selected tyrphostins causes cell cycle arrest at late G1 and S phase. Exp Cell Res, 1998, 241(2): 340-351.
[4]. Sevransky JE, Shaked G, Novogrodsky A, et al. Tyrphostin AG 556 improves survival and reduces multiorgan failure in canine Escherichia coli peritonitis. J Clin Invest, 1997, 99(8): 1966-1973.
| Physical Appearance | Yellow solid |
| Storage | Store at -20°C |
| M.Wt | 336.39 |
| Cas No. | 133550-41-1 |
| Formula | C20H20N2O3 |
| Solubility | <10.09mg/ml in DMSO |
| Chemical Name | (E)-2-cyano-3-(3,4-dihydroxyphenyl)-N-(4-phenylbutyl)acrylamide |
| SDF | Download SDF |
| Canonical SMILES | O=C(/C(C#N)=C/C(C=C1O)=CC=C1O)NCCCCC2=CC=CC=C2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
