AG 494
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AG 494是一种强效的和选择性的EGFR抑制剂,IC 50值为0.7 μM.
表皮生长因子受体(EGFR)是表皮生长因子的细胞表面受体,在肿瘤侵袭和癌细胞增殖中起重要作用.
AG 494是一种强效的和选择性的EGFR抑制剂.AG 494抑制EGFR和HER-2的自磷酸化,IC50值分别为1.1和39 μM.在DHER-14细胞中,AG 494抑制Cdk2的活化和EGF依赖性DNA合成[1].AG 494将细胞阻滞在晚G1期和S期,并通过磷酸化Cdk2上的酪氨酸15抑制Cdk2的活化[2].表皮生长因子(EGF)增加(骨形态发生蛋白)BMP9诱导的间质干细胞(MSCs)的成骨标记物,这被AG-494时间和剂量依赖性地抑制[3].在人前列腺(DU145)和肺(A549)癌细胞系中,AG494剂量依赖性地可逆地显著抑制自分泌生长.此外,在两种细胞系中,AG494诱导细胞凋亡并阻滞细胞生长在G1期[4].
参考文献：
[1]. Osherov N, Levitzki A. Tyrphostin AG 494 blocks Cdk2 activation. FEBS Lett, 1997, 410(2-3): 187-190.
[2]. Kleinberger-Doron N, Shelah N, Capone R, et al. Inhibition of Cdk2 activation by selected tyrphostins causes cell cycle arrest at late G1 and S phase. Exp Cell Res, 1998, 241(2): 340-351.
[3]. Liu X, Qin J, Luo Q, et al. Cross-talk between EGF and BMP9 signalling pathways regulates the osteogenic differentiation of mesenchymal stem cells. J Cell Mol Med, 2013, 17(9): 1160-1172.
[4]. Bojko A1, Reichert K, Adamczyk A, et al. The effect of tyrphostins AG494 and AG1478 on the autocrine growth regulation of A549 and DU145 cells. Folia Histochem Cytobiol, 2012, 50(2): 186-195.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 280.28 |
| Cas No. | 133550-35-3 |
| Formula | C16H12N2O3 |
| Solubility | Soluble in DMSO |
| Chemical Name | (E)-2-cyano-3-(3,4-dihydroxyphenyl)-N-phenylacrylamide |
| SDF | Download SDF |
| Canonical SMILES | OC1=CC(/C=C(C#N)/C(NC2=CC=CC=C2)=O)=CC=C1O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
