切换导航

ACSF

现货
Catalog No.
B5443
脑脊液的替代物
组合的产品项目
规格价格库存 数量
25ml
¥ 3,024.00
Ship with 10-15 days

电话: 021-55669583

邮箱: sales@apexbio.cn

全球经销商

Background

ACSF is often used as replacement of CSF for perfusion of brain slices to preserve interneurons [1].
ACSF (artificial cerebrospinal fluid) is invented to reduce the incidence of cerebral edema and further suppress brain cell disorders. And ACSF is often used as an irrigation fluid or perfusion fluid in the field of neurosurgery, such as intracranial surgery, or when used as a replenishing fluid for lost cerebrospinal fluid. ACSF is also used to maintain pH balance and tissue oxygen delivery for research [2, 3].
ACSF is often used to maintain oxygen supply in the study of basic characteristics of neuronal function where acute in vitro brain slice models are required. When tested with neocortical mouse slices, carbogenated ACSF treatment could keep pH balance and tissue oxygen delivery; otherwise, seizure-like events were occurred in the slices with no-magnesium ACSF treatment [1]. In the study of targeted drugs for protect neuron, ACSF is often to treat the mice and worked as a control to calculate the drug efficiency [3]
It is also reported in the rat brain slice model, treated with ACSF, a replacement of human CSF, could powerfully boost spontaneous firing of CA1, CA3 and layer 5 pyramidal neurons [4].
References:
[1].    Voss, L.J., S.A. George, and J.W. Sleigh, Testing neocortical slice viability in non-perfused no-magnesium artificial cerebrospinal fluid solutions. J Neurosci Methods, 2012. 204(2): p. 273-5.
[2].    Liu, D. and F. Bao, Hydrogen peroxide administered into the rat spinal cord at the level elevated by contusion spinal cord injury oxidizes proteins, DNA and membrane phospholipids, and induces cell death: Attenuation by a metalloporphyrin. Neuroscience, 2015. 285: p. 81-96.
[3].    Gruber, R.C., et al., Targeted GAS6 delivery to the CNS protects axons from damage during experimental autoimmune encephalomyelitis. J Neurosci, 2014. 34(49): p. 16320-35.
[4].    Bjorefeldt, A., et al., Human cerebrospinal fluid increases the excitability of pyramidal neurons in the in vitro brain slice. J Physiol, 2015. 593(1): p. 231-43.

Chemical Properties

Physical AppearanceColourless liquid
StorageStore at RT
M.Wt320.38
FormulaC20H20N2O2
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

质量控制

质量控制和MSDS

批次: