ABC294640
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ABC294640是选择性的和竞争性的鞘氨醇激酶2(SK2)抑制剂,其IC50值为60 μM[1].
ABC294640是SK2的竞争性抑制剂,对重组人SK2的IC50值约为60 μM.在动力学试验中,ABC294640与鞘氨醇竞争,其Ki值为9.8 μM.ABC294640靶向SK2的鞘氨醇结合位点,对其它20种激酶没有抑制活性.在MDA-MB-231细胞中,ABC294640抑制S1P生成,其IC50值为26 μM.ABC294640对肿瘤细胞具有抗增殖和抗迁移作用,可以抑制多种肿瘤细胞系的增殖,如1025LU\A-498\Caco-2和HT-29,IC50值范围为6 μM ~ 48 μM.在乳腺癌异种移植的免疫功能正常BALB/c小鼠中,ABC294640抑制肿瘤生长.据发现,相对于雌激素受体阴性乳腺癌细胞,ABC294640对雌激素受体阳性乳腺癌细胞具有更强的抗增殖作用.ABC294640对E2信号的影响有望用于治疗内分泌相关疾病[1,2].
参考文献：
[1] French K J, Zhuang Y, Maines L W, et al. Pharmacology and antitumor activity of ABC294640, a selective inhibitor of sphingosine kinase-2. Journal of Pharmacology and Experimental Therapeutics, 2010, 333(1): 129-139.
[2] Antoon J W, White M D, Meacham W D, et al. Antiestrogenic effects of the novel sphingosine kinase-2 inhibitor ABC294640. Endocrinology, 2010, 151(11): 5124-5135.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 380.91 |
| Cas No. | 915385-81-8 |
| Formula | C23H25ClN2O |
| Solubility | ≥38.1 mg/mL in DMSO; insoluble in H2O; ≥58.9 mg/mL in EtOH |
| Chemical Name | (7S)-3-(4-chlorophenyl)-N-(pyridin-4-ylmethyl)adamantane-1-carboxamide |
| SDF | Download SDF |
| Canonical SMILES | C1C2CC3(CC1CC(C2)(C3)C(=O)NCC4=CC=NC=C4)C5=CC=C(C=C5)Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
