A 286982
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
A 286982是LFA-1/ICAM-1结合及LFA-1的选择性抑制剂,IC50值分别为44 nM和35 nM [1].
淋巴细胞功能相关抗原-1(LFA-1)是白细胞整合素家族的一员,参与T细胞与抗原呈递细胞的结合.胞内粘附分子-1(ICAM-1)是胞内粘附分子的一种类型,在细胞与细胞相互作用中起重要作用.LFA-1/ICAM-1相互作用在炎症疾病的发病机制中发挥重要作用[2].
A 286982是高效的LFA-1/ICAM-1抑制剂,在化合物2B的结合中发挥不同作用.在LFA-1/ICAM-1结合实验和LFA-1介导的细胞粘附实验中,A 286982处理28小时能够抑制LFA-1/ICAM-1相互作用[1].LFA-1/ICAM-1 ELISA实验表明,A 286982通过降低二者配体亲和力与结合能力,从而抑制ICAM-1结合LFA-1 [3].
参考文献：
[1]. Liu, G., et al., Discovery of novel p-arylthio cinnamides as antagonists of leukocyte functionassociated antigen-1/intracellular adhesion molecule-1 interaction. 1. Identification of an additional binding pocket based on an anilino diaryl sulfide lead. J Med Chem, 2000. 43(21): p. 4025-40.
[2]. Katakai, T., K. Habiro, and T. Kinashi, Dendritic cells regulate high-speed interstitial T cell migration in the lymph node via LFA-1/ICAM-1. J Immunol, 2013. 191(3): p. 1188-99.
[3]. Keating, S.M., et al., Competition between intercellular adhesion molecule-1 and a smallmolecule antagonist for a common binding site on the alphal subunit of lymphocyte functionassociated antigen-1. Protein Sci, 2006. 15(2): p. 290-303.
| Physical Appearance | Yellow solid |
| Storage | Store at RT |
| M.Wt | 453.55 |
| Cas No. | 280749-17-9 |
| Formula | C24H27N3O4S |
| Solubility | ≥51.2 mg/mL in DMSO; ≥25.55 mg/mL in EtOH; insoluble in H2O |
| Chemical Name | (E)-1-(4-acetylpiperazin-1-yl)-3-[3-nitro-4-(2-propan-2-ylphenyl)sulfanylphenyl]prop-2-en-1-one |
| SDF | Download SDF |
| Canonical SMILES | CC(C)C1=CC=CC=C1SC2=C(C=C(C=C2)C=CC(=O)N3CCN(CC3)C(=O)C)[N+](=O)[O-] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
