5-Methyl-CTP
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
5-Methyl-CTP is a modified nucleoside triphosphate, commonly used to substitute the naturally occurring cytidine triphosphate (CTP) during in vitro mRNA synthesis. Methylation of the cytosine C-5 position is a common, post-transcriptional modification for mRNA. Incorporation of chemically modified 5-methyl-CTP can increase mRNA stability and protein expression, and meanwhile, reduce interaction of innate immune receptors with in vitro-transcribed (IVT) mRNA. Apart from its application in encoding antigens for the activation of immune response against a variety of diseases such as COVID-19, IVT mRNA can also express the nanobody/variable domain of heavy chain of heavy-chain antibody targeting the intracellular components, enabling intracellular antigen labeling and manipulation.
References:
1. Rahman MM, Zhou N, Huang J. An overview on the development of mRNA-based vaccines and their formulation strategies for improved antigen expression in vivo. Vaccines (Basel), 2021, 9(3): 244.
2. Lu J, Lu G, Tan S, et al. A COVID-19 mRNA vaccine encoding SARS-CoV-2 virus-like particles induces a strong antiviral-like immune response in mice. Cell Research, 2020, 30(10): 936-939.
3. Zhou X, Hao R, Chen C, et al. Rapid delivery of nanobodies/VHHs into living cells via expressing in vitro-transcribed mRNA. Molecular Therapy - Methods & Clinical Development, 2020, 17: 401-408.
- 1. Denzil Furtado, Christina Cortez-Jugo, et al. "mRNA Treatment Rescues Niemann-Pick Disease Type C1 in Patient Fibroblasts." Mol Pharm. 2022 Nov 7;19(11):3987-3999. PMID: 36125338
- 2. Yao Li, Xiaotu Ma, et al. "Rapid Surface Display of mRNA Antigens by BacteriaDerived Outer Membrane Vesicles for a Personalized Tumor Vaccine." Adv Mater. 2022 May;34(20):e2109984. PMID: 35315546
- 3. Jingjing Zhang , Yun Li, et al. "Genome-wide CRISPR/Cas9 library screen identifes PCMT1 as a critical driver of ovarian cancer metastasis." J Exp Clin Cancer Res. PMID: 35033172
- 4. Jing Lu, Guoliang Lu, et al. "A COVID-19 mRNA vaccine encoding SARS-CoV-2 virus-like particles induces a strong antiviral-like immune response in mice." Cell Res. 2020;1-4. PMID:32801356
Physical Appearance | Solution |
Storage | Store at -20°C or below |
M.Wt | 497.1 (free acid) |
Formula | C10H18N3O14P3 (free acid) |
Synonyms | 5-Methylcytidine-5'-Triphosphate, 5-Me-CTP, 5mCTP, 5-mCTP |
SDF | Download SDF |
Canonical SMILES | [NH2]=C(C(C)=CN1C2C(O)C(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O2)=NC1=O.[Li+].[Li+].[Li+].[Li+] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |