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5-BrdU

现货
Catalog No.
B3589
胸腺嘧啶核苷类似物
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 550.00
现货
500mg
¥ 500.00
现货
1g
¥ 800.00
现货

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Background

5-Bromodeoxyuridine (5-BrdU) is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by monoclonal antibodies or polyclona111.

In vitro: The effect of 5-BrdU on the proliferation was studied in the early phases of dedifferentiation of Nicotiana glauca pith explants grown. It was found to be wholly inhibitory only when given during the first 72 h of culture, this inhibition being reversed by simultaneous addition of either deoxyeytidine or thymidine [1].

In vivo: Young adult offspring mice were stained for 5-BrdU using a monoclonal antibody and the peroxidase-antiperoxidase method. The distribution of immunoreactive nuclei was very similar to the previous [3H]thymidine data. The pattern of labelled nuclei at different embryonic ages followed the spatiotemporal gradients described for cortical and hippocampal neurogenesis. These data indicate that BrdU can be used to map neuronal birthdates. [2].

Clinical trials: Currenlty no clinical data are available.

References:
[1] M.  Durante, C. Geri, V. Nuti-Ronchi, G. Martini, E. Guillé, J. Grisvard, L. Giorgi, R. Parenti, M. Bulatti. Inhibition of Nicotiana glauca pich tissue proliferation through incorporation of 5-BrdU into SNA. Cell Differentiation, Volume 6, Issue 1, June 1977, Pages 53–63
[2] del Rio JA, Soriano E.   Immunocytochemical detection of 5'-bromodeoxyuridine incorporation in the central nervous system of the mouse. Brain Res Dev Brain Res. 1989 Oct 1;49(2):311-7.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt307.1
Cas No.59-14-3
FormulaC9H11BrN2O5
Solubility≥15.35mg/mL in DMSO, ≥16.23 mg/mL in H2O with ultrasonic, ≥11.56 mg/mL in 0.9% NS with ultrasonic
Chemical Name5-bromo-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione
SDFDownload SDF
Canonical SMILESBrC(C(N1)=O)=CN(C1=O)[C@@]2([H])C[C@@](O)([H])[C@@](O2)([H])CO
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

体外实验 [1]:

样本

烟草髓组织

溶解方法

该化合物在DMSO中的溶解度>15.4mg/mL。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。

反应条件

5 μg/ml; 24 h

应用

在烟碱髓组织中,BrdU抑制了髓外植体的生长,而胸苷完全逆转了抑制作用。观察到的抑制作用可归因于BrdU掺入DNA。

动物实验[2]:

动物模型

怀孕的NMRI小鼠

剂量

25 mg/kg (5 mg/ml 溶于0.1 M Tris缓冲盐水中,pH 7.6),腹腔注射; 胚胎期E11和E17之间。

应用

在怀孕的NMRI小鼠中,在胚胎期E11和E17之间注射单次脉冲的5-bromodeoxyuridine (BrdU)以标记有丝分裂后神经母细胞。BrdU是Thy的类似物,能够插入经历S期的细胞的DNA中,并且可以通过多克隆或单克隆抗体检测。在BrdU脉冲后,在成人CNS中检测到免疫反应性核。BrdU可用于映射神经元的出生日期。

注意事项

请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同,这是由实验系统的误差引起的,属于正常现象。

References:

[1] M. Durante, C. Geri, V. Nuti-Ronchi, G. Martini, E. Guillé, J. Grisvard, L. Giorgi, R. Parenti, M. Bulatti. Inhibition of Nicotiana glauca pich tissue proliferation through incorporation of 5-BrdU into SNA. Cell Differentiation, Volume 6, Issue 1, June 1977, Pages 53–63

[2] del Rio JA, Soriano E. Immunocytochemical detection of 5'-bromodeoxyuridine incorporation in the central nervous system of the mouse. Brain Res Dev Brain Res. 1989 Oct 1;49(2):311-7.

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