Δ4-Dafachronic Acid
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Δ4-Dafachronic acid is a DAF-12 activator.
Steroid hormone dafachronic acid (DA) has been identified to regulate dauer formation and lifespan in Caenorhabditis elegans via binding to the nuclear receptor DAF-12. DAF-12, an orphan nuclear hormone receptor, regulates dauer larva diapause, reproductive development, fat metabolism, as well as life cycle/longevity in C. elegans. In addition, DAF-12 is needed for entry into dauer by acting as a transcriptional repressor of target genes that favor reproductive development when it is not bound to ligand.
In vitro: Δ4-Dafachronic acid is a racemic version of a sterol-derived hormone acting as a ligand of DAF-12. In a previous study, it was found that there was no significant increase in the Δ4- and Δ4-dafachronic acid concentration in the long-lived glp-1 mutant, nor in the mutants defective in dauer formation [1]. Another previous study provided insights into the molecular interface between DA and IIS pathways and suggested that Δ4- and Δ7-dafachronic acid pathways had unique as well as overlapping biological functions in the control of development and lifespan [2].
In vivo: Up to now, there is no animal in vivo study reported for Δ4-Dafachronic acid.
Clinical trial: So far, no clinical study has been conducted.
References:
[1] Li TM et al. No Significant Increase in the Δ4- and Δ7-Dafachronic Acid Concentration in the Long-Lived glp-1 Mutant, nor in the Mutants Defective in Dauer Formation. G3 (Bethesda). 2015 May 12;5(7):1473-9.
[2] Dumas, K. J.,Guo, C.,Wang, X., et al. Functional divergence of dafachronic acid pathways in the control of C. elegans development and lifespan. Dev.Biol. 340(2), 605-612 (2010).
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 414.6 |
Cas No. | 23017-97-2 |
Formula | C27H42O3 |
Synonyms | 3-keto-4-Cholestenoic Acid |
Solubility | ≤12.5mg/ml in ethanol;2.5mg/ml in DMSO;5mg/ml in dimethyl formamide |
Chemical Name | 3-oxo-cholest-4-en-26-oic acid |
SDF | Download SDF |
Canonical SMILES | O=C1CC[C@@]2(C)C(CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@@H]4[C@@H](CCCC(C)C(O)=O)C)=C1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
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