3-Nitropropionic acid
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
3-Nitropropionic acid is an irreversible inhibitor of mitochondrial respiratory complex II (succinate dehydrogenase), which impairs cellular energy metabolism by inhibiting succinate dehydrogenase and reducing ATP production. Also, 3-nitropropionic acid can cause the generation and release of reactive oxygen species (ROS) from mitochondria (the main source of cellular oxidative stress), mitochondrial DNA damage, as well as loss of mitochondrial function.
References:
1. Huang LS, Sun G, Cobessi D, et al. 3-Nitropropionic acid is a suicide inhibitor of mitochondrial respiration that, upon oxidation by complex II, forms a covalent adduct with a catalytic base arginine in the active site of the enzyme. Journal of Biological Chemistry, 2006, 281(9): 5965-5972.
2. Zhang JQ, Shen M, Zhu CC, et al. 3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse. PLoS One, 2014, 9(2): e86589.
3. Mandavilli BS, Boldogh I, Van Houten B. 3-Nitropropionic acid-induced hydrogen peroxide, mitochondrial DNA damage, and cell death are attenuated by Bcl-2 overexpression in PC12 cells. Brain research. Molecular brain research, 2005, 133(2): 215-223.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 119.08 |
Cas No. | 504-88-1 |
Formula | C3H5NO4 |
Solubility | ≥103 mg/mL in H2O; ≥114.2 mg/mL in EtOH; ≥6 mg/mL in DMSO |
Chemical Name | 3-nitropropanoic acid |
SDF | Download SDF |
Canonical SMILES | OC(CC[N+]([O-])=O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Cell experiment:[3] | |
Cell lines |
PC12 cells |
Reaction Conditions |
4 or 8 mM 3-nitropropionic acid for 48 h incubation |
Applications |
3-Nitropropionic acid induced cytotoxicity by triggering ROS production and mitochondrial DNA damage, which could be avoided by overexpressing Bcl-2 in PC12 cells. |
Animal experiment:[2] | |
Animal models |
Female ICR mice |
Dosage form |
6.25, 12.5 and 25 mg/kg Continuous intraperitoneal injection for 7 days |
Applications |
3-Nitropropionic acid induced granulosa cell apoptosis, large follicle atresia, and an increase of ROS levels in the ovary. Thus, 3-nitropropionic acid could be used to establish an in vivo model of ovarian oxidative stress for studying the mechanism of resulting damage induced by free radicals and for the screening of novel antioxidants. |
Note |
The technical data provided above is for reference only. |
References: 1. Huang LS, Sun G, Cobessi D, et al. 3-Nitropropionic acid is a suicide inhibitor of mitochondrial respiration that, upon oxidation by complex II, forms a covalent adduct with a catalytic base arginine in the active site of the enzyme. Journal of Biological Chemistry, 2006, 281(9): 5965-5972. 2. Zhang JQ, Shen M, Zhu CC, et al. 3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse. PLoS One, 2014, 9(2): e86589. 3. Mandavilli BS, Boldogh I, Van Houten B. 3-Nitropropionic acid-induced hydrogen peroxide, mitochondrial DNA damage, and cell death are attenuated by Bcl-2 overexpression in PC12 cells. Brain research. Molecular brain research, 2005, 133(2): 215-223. |
质量控制和MSDS
- View current batch:
-
Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)