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3-Nitropropionic acid

3-硝基丙酸
Catalog No.
B5734
线粒体呼吸复合物II(琥珀酸脱氢酶)抑制剂
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 454.00
现货
50mg
¥ 318.00
现货

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背景

3-Nitropropionic acid is an irreversible inhibitor of mitochondrial respiratory complex II (succinate dehydrogenase), which impairs cellular energy metabolism by inhibiting succinate dehydrogenase and reducing ATP production. Also, 3-nitropropionic acid can cause the generation and release of reactive oxygen species (ROS) from mitochondria (the main source of cellular oxidative stress), mitochondrial DNA damage, as well as loss of mitochondrial function.

References:

1. Huang LS, Sun G, Cobessi D, et al. 3-Nitropropionic acid is a suicide inhibitor of mitochondrial respiration that, upon oxidation by complex II, forms a covalent adduct with a catalytic base arginine in the active site of the enzyme. Journal of Biological Chemistry, 2006, 281(9): 5965-5972.

2. Zhang JQ, Shen M, Zhu CC, et al. 3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse. PLoS One, 2014, 9(2): e86589.

3. Mandavilli BS, Boldogh I, Van Houten B. 3-Nitropropionic acid-induced hydrogen peroxide, mitochondrial DNA damage, and cell death are attenuated by Bcl-2 overexpression in PC12 cells. Brain research. Molecular brain research, 2005, 133(2): 215-223.

化学属性

Physical AppearanceA solid
StorageStore at -20°C
M.Wt119.08
Cas No.504-88-1
FormulaC3H5NO4
Solubility≥103 mg/mL in H2O; ≥114.2 mg/mL in EtOH; ≥6 mg/mL in DMSO
Chemical Name3-nitropropanoic acid
SDFDownload SDF
Canonical SMILESOC(CC[N+]([O-])=O)=O
运输条件 蓝冰运输或根据您的需求运输。
一般建议不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。

试验操作

Cell experiment:[3]

Cell lines

PC12 cells

Reaction Conditions

4 or 8 mM 3-nitropropionic acid for 48 h incubation

Applications

3-Nitropropionic acid induced cytotoxicity by triggering ROS production and mitochondrial DNA damage, which could be avoided by overexpressing Bcl-2 in PC12 cells.

Animal experiment:[2]

Animal models

Female ICR mice

Dosage form

6.25, 12.5 and 25 mg/kg

Continuous intraperitoneal injection for 7 days

Applications

3-Nitropropionic acid induced granulosa cell apoptosis, large follicle atresia, and an increase of ROS levels in the ovary. Thus, 3-nitropropionic acid could be used to establish an in vivo model of ovarian oxidative stress for studying the mechanism of resulting damage induced by free radicals and for the screening of novel antioxidants.

Note

The technical data provided above is for reference only.

References:

1. Huang LS, Sun G, Cobessi D, et al. 3-Nitropropionic acid is a suicide inhibitor of mitochondrial respiration that, upon oxidation by complex II, forms a covalent adduct with a catalytic base arginine in the active site of the enzyme. Journal of Biological Chemistry, 2006, 281(9): 5965-5972.

2. Zhang JQ, Shen M, Zhu CC, et al. 3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse. PLoS One, 2014, 9(2): e86589.

3. Mandavilli BS, Boldogh I, Van Houten B. 3-Nitropropionic acid-induced hydrogen peroxide, mitochondrial DNA damage, and cell death are attenuated by Bcl-2 overexpression in PC12 cells. Brain research. Molecular brain research, 2005, 133(2): 215-223.

质量控制

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化学结构

3-Nitropropionic acid