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3-Methyladenine

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Catalog No.
A8353
III类PI3K抑制剂
组合的产品项目
规格价格库存 数量
10mM (in 1mL DMSO)
¥ 500.00
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50mg
¥ 500.00
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200mg
¥ 1,200.00
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500mg
¥ 2,400.00
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Background

3-Methyladenine is an inhibitor of class III phosphoinositide 3-kinase (PI3K) [1].

3-Methyladenine is widely used as an autophagy inhibitor through inhibiting class III PI3K without significantly affecting protein synthesis or ATP levels. 3-Methyladenine also inhibits class I PI3K. It is found that 3-Methyladenine has different effects on class I and class III PI3K. It blocks class I PI3K persistently while inhibits class III PI3K transiently. As an inhibitor of autophagy, 3-Methyladenine is usually used to study the role of autophagy. In addition, 3-Methyladenine is also found to have anti-cancer efficacy. It induces cell death of tumor under nutrient-starved conditions. In HT1080 cells, 3-Methyladenine significantly inhibits cell migration through reducing membrane ruffle and lamellipodia formation in normal culture condition. It also inhibits the invasion of HT1080 cells [1, 2].

References:
[1] Wu Y T, Tan H L, Shui G, et al. Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. Journal of Biological Chemistry, 2010, 285(14): 10850-10861.
[2] Ito S, Koshikawa N, Mochizuki S, et al. 3-Methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinases independently of autophagy inhibition. International journal of oncology, 2007, 31(2): 261-268.

文献引用

1. Tang RH, Qi RQ, et al. "Interleukin-4 affects microglial autophagic flux." Neural Regen Res. 2019 Sep;14(9):1594-1602. PMID:31089059
2. Sarah Needs. "The Biochemical Basis of Congenital Disorders of Glycosylation." The Open University. 2019.
3. Gierisch ME, Pfistner F, et al. "Proteasomal Degradation of the EWS-FLI1 Fusion Protein Is Regulated by a Single Lysine Residue." J Biol Chem. 2016 Dec 23;291(52):26922-26933. PMID:27875302
4.Xue H, Yuan G, Guo X, et al. "A Novel Tumor-promoting Mechanism of IL6 and the Therapeutic Efficacy of Tocilizumab: Hypoxia-induced IL6 Is a Potent Autophagy Initiator in Glioblastoma via the p-STAT3-MIR155-3p-CREBRF Pathway[J]." Autophagy, 10 May 2016.
Physical AppearanceA solid
StorageStore at -20°C
M.Wt149.15
Cas No.5142-23-4
FormulaC6H7N5
Synonyms3-MA
Solubility≥7.45mg/mL in DMSO, ≥5mg/mL in H2O
Chemical Name3-methylpurin-6-amine
SDFDownload SDF
Canonical SMILESCN1C=NC(=C2C1=NC=N2)N
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。
细胞实验 [1]:

细胞系

HT1080细胞

制备方法

在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液,一般步骤如下:请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。

反应条件

5或10 mM;10小时

实验结果

3-Methyladenine (3-MA) (5 ~ 10 mM) 有效抑制HT1080细胞迁移。此外,与100 nM WMN和25 μM LY294002相比,10 mM 3-MA更能有效抑制HT1080细胞迁移。

References:

[1]. Ito S, Koshikawa N, Mochizuki S, et al. 3-Methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinases independently of autophagy inhibition. International journal of oncology, 2007, 31(2): 261-268.

描述 3-Methyladenine是一种选择性的PI3K抑制剂,作用于Vps34和PI3Kγ,IC50值分别为25 μM和60 μM。
靶点 Vps34 PI3Kγ        
IC50 25 μM 60 μM        

化学结构

3-Methyladenine

相关生物数据

3-Methyladenine
In Western blot assay, we test whether Wortmannin and 3-Me could inhibit the effect of autophagy in 3T3 cells. The 1mM of inhibitors can suppress the cell survival of 3T3 cells. Due to the death of cell at the high concentration of Wortmannin and 3-Me, they are not fit to test in Western blot assay. The result has been shown that the low concentration of Wortmannin and 3-Me (1μM) significantly enhanced the P62 and inhibited the level of autophagy in 3T3 cells.
Method:Western blot; Cell Lines:3T3 cell lines; Concentrations:1μM~1mM; Incubation Time:24 h.

相关生物数据

3-Methyladenine

相关生物数据

3-Methyladenine

相关生物数据

3-Methyladenine

相关生物数据

3-Methyladenine