3-Methyladenine
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
3-Methyladenine是III类磷酸肌醇3-激酶（PI3K）的抑制剂[1]。
3-Methyladenine通过抑制III类PI3K被广泛用作自噬抑制剂，而对蛋白合成或ATP水平没有显著影响。3-Methyladenine也可以抑制I类PI3K。据报道，3-Methyladenine对I类和III类PI3K具有不同的效应，持续阻断I类PI3K，而仅瞬时抑制III类PI3K。作为自噬的抑制剂，3-Methyladenine通常被用于研究自噬作用。此外，3-Methyladenine具有抗癌活性，在营养饥饿条件下诱导肿瘤细胞死亡。在HT1080细胞中，3-Methyladenine通过减少膜皱褶和伪足的形成，从而显著抑制细胞迁移。3-Methyladenine也可以抑制HT1080细胞的侵袭[1，2]。
参考文献：
[1] Wu Y T, Tan H L, Shui G, et al. Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. Journal of Biological Chemistry, 2010, 285(14): 10850-10861.
[2] Ito S, Koshikawa N, Mochizuki S, et al. 3-Methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinases independently of autophagy inhibition. International journal of oncology, 2007, 31(2): 261-268.
- 1. Dejuan Sun, Yueying Yang, et al. "Identification of ADP-ribosylation factor 6 as the cellular target of withangulatin A against TNBC cells by ferroptosis." Research Square. October 14th, 2022.
- 2. Yuanhui Zhu, Xi Wang, et al. "Targeting Aβ and p-Tau Clearance in Methamphetamine-Induced Alzheimer's Disease-Like Pathology: Roles of Syntaxin 17 in Autophagic Degradation in Primary Hippocampal Neurons." Oxid Med Cell Longev. 2022 May 18;2022:3344569. PMID: 35633882
- 3. Shuyi Chen, Yabiao Gao, et al. "Anti-cancer Drug Anlotinib Promotes Autophagy and Apoptosis in Breast Cancer." Front Biosci (Landmark Ed). 2022 Apr 7;27(4):125. PMID: 35468684
- 4. Du Yu, Yundi Zhao, et al. "C19orf66 Inhibits Japanese Encephalitis Virus Replication by Targeting -1 PRF and the NS3 Protein." Virol Sin. 2021 Dec;36(6):1443-1455. PMID: 34309824
- 5. Chenghao Li, Jiawei Li, et al. "Isorhamnetin Promotes MKN-45 Gastric Cancer Cell Apoptosis by Inhibiting PI3K-Mediated Adaptive Autophagy in a Hypoxic Environment." J Agric Food Chem. 2021 Jul 28;69(29):8130-8143. PMID: 34269571
- 6. Renjie Dou, Jinjun Qian, et al. "Suppression of steroid 5α-reductase type I promotes cellular apoptosis and autophagy via PI3K/Akt/mTOR pathway in multiple myeloma." Cell Death Dis. 2021 Feb 24;12(2):206. PMID: 33627630
- 7. Xiaoli Liu, Yan Gu, et al. "Honokiol induces paraptosis-like cell death of acute promyelocytic leukemia via mTOR & MAPK signaling pathways activation." Apoptosis. 2021 Apr;26(3-4):195-208. PMID: 33550458
- 8. Shichao Lv, Peng Yuan, et al. "QiShenYiQi pill improves the reparative myocardial fibrosis by regulating autophagy." J Cell Mol Med 2020 Sep 2. PMID: 32881330
- 9. Baicun Li, Jie Yao, et al. "Design, synthesis, and biological evaluation of 5-((8-methoxy-2-methylquinolin-4-yl)amino)-1H-indole-2-carbohydrazide derivatives as novel Nur77 modulators." Eur J Med Chem. 2020 Oct 15;204:112608. PMID: 32717483
- 10. Shuyi Chen, Ping Zhu, et al. "Anlotinib Inhibits the Growth of Breast Cancer Cells by Promoting Autophagy and Apoptosis via the Akt/GSK-3α Signalling Pathway." Research Square. October 9th, 2020.
- 11. HAIDONG SUN, HUAJIE ZONG, et al. " 2‑Hydroxypropyl‑β‑cyclodextrin blocks autophagy flux and triggers caspase‑8‑mediated apoptotic cascades in HepG2 cells." Mol Med Rep. 2020 Sep;22(3):1901-1909. PMID: 32705246
- 12. Meng XL, Fu P, et al. "Increased EZH2 Levels in Anterior Cingulate Cortex Microglia Aggravate Neuropathic Pain by Inhibiting Autophagy Following Brachial Plexus Avulsion in Rats." Neurosci Bull. 2020;10.1007/s12264-020-00502-w. PMID: 32346844
- 13. Zhao F, Wang J, et al. "Neuroprotection by Walnut-derived Peptides through Autophagy Promotion via Akt/mTOR Signaling Pathway against Oxidative Stress in PC12 Cells." J Agric Food Chem. 2020;10.1021/acs.jafc.9b08252. PMID: 32090563
- 14. Zhang H, He Y, et al."miR-30-5p-mediated ferroptosis of trophoblasts is implicated in the pathogenesis of preeclampsia." Redox Biol. 2020 Jan;29:101402. PMID: 31926626
- 15. Zhou H, Du R, et al. "Cannabinoid receptor 2 promotes the intracellular degradation of HMGB1 via the autophagy-lysosome pathway in macrophage." Int Immunopharmacol. 2020;78:106007. PMID: 31806570
- 16. Chen Y, Zhu E, et al. "Important roles of C-terminal residues in degradation of capsid protein of classical swine fever virus." Virol J. 2019 Nov 6;16(1):127. PMID: 31694654
- 17. Ye J, Xue M, et al. "Diosbulbin B-Induced Mitochondria-Dependent Apoptosis in L-02 Hepatocytes is Regulated by Reactive Oxygen Species-Mediated Autophagy." Front Pharmacol. 2019 Jun 19;10:676. PMID: 31275148
- 18. Tang RH, Qi RQ, et al. "Interleukin-4 affects microglial autophagic flux." Neural Regen Res. 2019 Sep;14(9):1594-1602. PMID: 31089059
- 19. Sarah Needs. "The Biochemical Basis of Congenital Disorders of Glycosylation." The Open University. 2019.
- 20. Gierisch ME, Pfistner F, et al. "Proteasomal Degradation of the EWS-FLI1 Fusion Protein Is Regulated by a Single Lysine Residue." J Biol Chem. 2016 Dec 23;291(52):26922-26933. PMID: 27875302
- 21. Xue H, Yuan G, Guo X, et al. "A Novel Tumor-promoting Mechanism of IL6 and the Therapeutic Efficacy of Tocilizumab: Hypoxia-induced IL6 Is a Potent Autophagy Initiator in Glioblastoma via the p-STAT3-MIR155-3p-CREBRF Pathway[J]." Autophagy, 10 May 2016.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 149.15 |
| Cas No. | 5142-23-4 |
| Formula | C6H7N5 |
| Synonyms | 3-MA |
| Solubility | ≥5 mg/mL in H2O; ≥7.45 mg/mL in DMSO; ≥8.97 mg/mL in EtOH |
| Chemical Name | 3-methylpurin-6-amine |
| SDF | Download SDF |
| Canonical SMILES | CN1C=NC(=C2C1=NC=N2)N |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
|
细胞系 |
HT1080细胞 |
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制备方法 |
在DMSO中的溶解度大于10 mM。若配制更高浓度的溶液，一般步骤如下：请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。 |
|
反应条件 |
5或10 mM；10小时 |
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实验结果 |
3-Methyladenine (3-MA) (5 ~ 10 mM) 有效抑制HT1080细胞迁移。此外，与100 nM WMN和25 μM LY294002相比，10 mM 3-MA更能有效抑制HT1080细胞迁移。 |
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References: [1]. Ito S, Koshikawa N, Mochizuki S, et al. 3-Methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinases independently of autophagy inhibition. International journal of oncology, 2007, 31(2): 261-268. |
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| 描述 | 3-Methyladenine是一种选择性的PI3K抑制剂，作用于Vps34和PI3Kγ，IC50值分别为25 μM和60 μM。 | |||||
| 靶点 | Vps34 | PI3Kγ | ||||
| IC50 | 25 μM | 60 μM | ||||
质量控制和MSDS
- 批次:
化学结构
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