2-APB
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
2-APB是Ins(1,4,5)P3诱导Ca2+释放的拮抗剂，其IC50值为42 μM[1]。
Myo-Ins(1,4,5)P3受体（IP3R）介导内部Ca2+动员。IP3R参与钙波和钙振荡的产生。
2-APB是Ins(1,4,5)P3诱导Ca2+释放的拮抗剂。2-APB抑制Ins(1,4,5)P3诱导的大鼠小脑微粒体Ca2+释放，其IC50值为42 μM。在人类血小板和中性粒细胞中，将2-APB加入到细胞外液中可以抑制凝血酶诱导的细胞内Ca2+增加。此外，2APB抑制血管紧张素Ⅱ（AⅡ）诱导的胸主动脉收缩[1]。在HEK-293细胞中，细胞外的2-APB阻断人TRPC5通道，其IC50值为20 μM。此外，2-APB阻断TRPC6和TRPC3通道。在过度表达TRPC5的细胞中，2-APB抑制细胞增殖[2]。在小鼠胰腺腺泡细胞中，低浓度的2-APB可以显著抑制钙库调控的钙内流。在透化的腺泡细胞中，高浓度的2-APB抑制直接刺激的Ca2+释放和InsP3诱导的Ca2+释放[3]。
在I/R引起睾丸损伤的大鼠中，2-APB显著增加超氧化物歧化酶（SOD）、总抗氧化能力（TAC）和谷胱甘肽（GSH），同时减少丙二醛（MDA）和DNA片段，这表明2-APR具有抗凋亡和抗氧化作用[4]。
参考文献：
[1]. Maruyama T, Kanaji T, Nakade S, et al. 2APB, 2-aminoethoxydiphenyl borate, a membrane-penetrable modulator of Ins(1,4,5)P3-induced Ca2+ release. J Biochem, 1997, 122(3): 498-505.
[2]. Xu SZ, Zeng F, Boulay G, et al. Block of TRPC5 channels by 2-aminoethoxydiphenyl borate: a differential, extracellular and voltage-dependent effect. Br J Pharmacol, 2005, 145(4): 405-414.
[3]. Choi KJ, Kim KS, Kim SH, et al. Caffeine and 2-Aminoethoxydiphenyl Borate (2-APB) Have Different Ability to Inhibit Intracellular Calcium Mobilization in Pancreatic Acinar Cell. Korean J Physiol Pharmacol, 2010, 14(2): 105-111.
[4]. Sari E, Aksit H, Erken HA, et al. Protective effect of 2-APB on testicular ischemia-reperfusion injury in rats. J Urol, 2015, 193(3): 1036-1041.
- 1. Yingying Zhang, Jiaqi Tang, et al. "Prenatal hypoxia induced ETBR activation and abnormal ROS signalling in pulmonary artery cells of rat offspring." Reprod Toxicol. 2021 Oct;105:91-100. PMID: 34478853
- 2. Yinghui Cui, Fenglan Chu, et al. "Role of serosal TRPV4-constituted SOCE mechanism in secretagogues-stimulated intestinal epithelial anion secretion." Front Pharmacol. 2021 Jul 14;12:684538. PMID: 34335254
- 3. Hong F, Wu S, et al. "TRPM7 Upregulate the Activity of SMAD1 through PLC Signaling Way to Promote Osteogenesis of hBMSCs." Biomed Res Int. 2020;2020:9458983. PMID: 32596398
| Physical Appearance | A solid |
| Storage | Store at RT |
| M.Wt | 225.1 |
| Cas No. | 524-95-8 |
| Formula | C14H16BNO |
| Solubility | insoluble in H2O; ≥27.85 mg/mL in EtOH; ≥9.4 mg/mL in DMSO |
| Chemical Name | 2-((diphenylboryl)oxy)ethanamine |
| SDF | Download SDF |
| Canonical SMILES | NCCOB(C1=CC=CC=C1)C2=CC=CC=C2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
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Cell lines |
Mouse pancreatic acinar cells |
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Reaction Conditions |
10 ~ 100 μM 2-APB |
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Applications |
In mouse pancreatic acinar cells, 2-APB significantly inhibited store-operated Ca2+ (SOC) channel-mediated Ca2+ entry at low concentrations (~ 30 μM). At higher concentrations (~ 100 μM), 2-APB exerted multiple paradoxical effects including inhibition of InsP3-induced Ca2+ release and direct stimulation of Ca2+ release. |
| Animal experiment:[2] | |
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Animal models |
Adult male Sprague-Dawley rats, 250 ~ 300 g |
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Dosage form |
2 or 4 mg/kg Administered intraperitoneally |
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Applications |
In rats with ischemia-reperfusion (I/R) induced testicular injury, 2-APB significantly increased mean levels of superoxide dismutase, total antioxidant capacity and glutathione, and reduced mean malondialdehyde and DNA fragmentation levels, which suggested the antiapoptotic and antioxidative effects of 2-APB. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Choi KJ, Kim KS, Kim SH, et al. Caffeine and 2-aminoethoxydiphenyl borate (2-APB) have different ability to inhibit intracellular calcium mobilization in pancreatic acinar cell. The Korean Journal of Physiology & Pharmacology, 2010, 14(2): 105-111. 2. Sari E, Aksit H, Erken HA, et al. Protective effect of 2-APB on testicular ischemia-reperfusion injury in rats. The Journal of Urology, 2015, 193(3): 1036-1041. |
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质量控制和MSDS
- 批次:
化学结构
相关生物数据
相关生物数据
