2,4-Diacetylphloroglucinol
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
2,4-Diacetylphloroglucinol is an agent against various plant pathogenic fungi, viruses, bacteria, and nematodes.
2,4-Diacetylphloroglucinol, a non-nitrogen-containing compound produced by some isolates of P. fluorescens, has been used to increase crop yield by controlling plant root diseases.
In vitro: A previous study was conducted to determine if 2,4-diacetylphloroglucinol was toxic to selected nematodes. The plant-parasitic nematodes including Pratylenchus scribneri and Xiphinema americanum, Heterodera glycines, Meloidogyne incognita, and the bacterial-feeding nematodes such as Pristionchus pacificus, Caenorhabditis elegans, and Rhabditis rainai, were found to be immersed in concentrations ranging from 0 to 100 μg/ml of 2,4-diacetylphloroglucinol. Moreove, the egg hatch and viability of juveniles and adults were determined, and the results showed that 2,4-diacetylphloroglucinol was toxic to X. americanum adults with an LD50 of 8.3 μg/ml. 2,4-Diacetylphloroglucinol could decrease M. incognita egg hatch, but stimulated C. elegans hatch. Whereas, the viability of M. incognita J2 and of C. elegans J1 and adults was not affected. There were no observed effects on the other nematodes. These findings indicated that 2,4-diacetylphloroglucinol was not toxic to all nematodes, and did not affect the tested species of beneficial bacterial-feeding nematodes [1].
In vivo: Up to now, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] Meyer, S. L.F.,Halbrendt, J.M.,Carta, L.K., et al. Toxicity of 2,4-diacetylphloroglucinol (DAPG) to plant-parasitic and bacterial-feeding nematodes. Journal of Nematology 41(4), 274-280 (2009).
Storage | Store at -20°C |
M.Wt | 210.2 |
Cas No. | 2161-86-6 |
Formula | C10H10O5 |
Synonyms | 5-Acetyl-2,4,6-trihydroxyacetophenone,DAPG,1,3-Diacetyl-2,4,6-trihydroxybenzene |
Solubility | insoluble in H2O; ≥11.55 mg/mL in DMSO; ≥31.55 mg/mL in EtOH |
Chemical Name | 1,1'-(2,4,6-trihydroxy-1,3-phenylene)bis-ethanone |
SDF | Download SDF |
Canonical SMILES | OC1=C(C(C)=O)C(O)=C(C(C)=O)C(O)=C1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1]: | |
细胞系 |
秀丽隐杆线虫 |
溶解方法 |
在DMSO中的溶解度≥11.55mg/ml。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
反应条件 |
10 μg/ml 和75 μg/ml |
应用 |
在DAPG中孵育1和3小时会刺激秀丽隐杆线虫卵孵化。例如,在1小时时,在0、10和75μg/ ml DAPG的卵孵化率分别为2.8%,4.5%和9.1%。 |
References: [1]. Meyer SL, Halbrendt JM, Carta LK, Skantar AM, Liu T, Abdelnabby HM, Vinyard BT. Toxicity of 2,4-diacetylphloroglucinol (DAPG) to plant-parasitic and bacterial-feeding nematodes. J Nematol. 2009 Dec;41(4):274-80. PubMed PMID: 22736826; PubMed Central PMCID: PMC3381463. |