17-ODYA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
17 ODYA是一种强效的细胞色素P450脂肪酸ω羟化酶抑制剂[1].
ω羟化酶是细胞色素P450超家族酶的成员,是参与类固醇\胆固醇和其他脂类合成的单加氧酶.ω-羟化酶灭活并降解一种强效的炎症介质白三烯B4.
在大鼠门静脉分离的单细胞中,17-ODYA(5 μM)抑制延迟整流电流(IK(V)),但不影响A型电流(IK(A)).此外,17-ODYA(5 μM)通过钙敏感性钾通道(BKCa)增加电流[2].
在大鼠肾皮质微粒体中,17-ODYA抑制环氧二十碳三酸\20-羟-二十烷四烯酸和二羟基二十碳三酸的形成,IC50值小于100 nM.17-ODYA抑制61.3%的花生四烯酸的ω-羟基化并增加钠排泄和尿流量[1].在内皮完整(E +)和内皮去除(E-)的兔动脉中,17-ODYA提高血管紧张素II的疗效.此外,17-ODYA抑制乙酰胆碱松弛.17-ODYA通过抑制前列腺素ω羟化酶,从而改善血管收缩剂环氧合酶-2的代谢物释放[3].
参考文献:
[1]. Zou AP, Ma YH, Sui ZH, et al. Effects of 17-octadecynoic acid, a suicide-substrate inhibitor of cytochrome P450 fatty acid omega-hydroxylase, on renal function in rats. J Pharmacol Exp Ther, 1994, 268(1): 474-481.
[2]. Edwards G, Zygmunt PM, Hgesttt ED, et al. Effects of cytochrome P450 inhibitors on potassium currents and mechanical activity in rat portal vein. Br J Pharmacol, 1996, 119(4): 691-701.
[3]. Jerez S, Sierra L, de Bruno MP. 17-Octadecynoic acid improves contractile response to angiotensin II by releasing vasocontrictor prostaglandins. Prostaglandins Other Lipid Mediat, 2012, 97(1-2): 36-42.
Physical Appearance | A crystalline solid |
Storage | Store at RT |
M.Wt | 280.45 |
Cas No. | 34450-18-5 |
Formula | C18H32O2 |
Solubility | ≥89.8 mg/mL in DMSO; ≥9.84 mg/mL in EtOH |
Chemical Name | octadec-17-ynoic acid |
SDF | Download SDF |
Canonical SMILES | OC(CCCCCCCCCCCCCCCC#C)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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