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10Panx

现货
Catalog No.
A2700
Panx-1模拟抑制肽
组合的产品项目
规格价格库存 数量
1mg
¥ 500.00
现货
5mg
¥ 1,000.00
现货
10mg
¥ 1,800.00
现货
25mg
¥ 4,000.00
现货

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Background

10Panx is a mimetic inhibitory peptide of panx1, which is a mammalian protein ectopically expressed to function as a hemi-channel, that readily and reversibly inhibits the panx1 currents. 10Panx has attracted increasing attention as it allows scientists readily and reliably to examine inhibition of panx1 in cells where efficient and consistent delivery of siRNA is highly difficult. According to results of multiple previous studies, 10Panx selectively inhibits P2X7R-induced dye uptake without altering other aspects of R2X7R activation and ATP-evoked membrane currents, constitutive dye-uptake and hemichannel-like currents that are induced by the overexpression of panx1, and ATP-mediated IL-1β release.

Reference

Pablo Pelegrin and Annmarie Surprenant. Pannexin-1 mediates large pore formation and interleukin-1β release by the ATP-gated P2X7 receptor. The EMBO Journal (2006) 25, 5071-5082

文献引用

1. Gao J, Zhang T, et al. "The F0F1 ATP synthase regulates human neutrophil migration through cytoplasmic proton extrusion coupled with ATP generation." Mol Immunol. 2017 Aug 23;90:219-226. PMID:28843171

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt1242.37
Cas No.955091-53-9
FormulaC58H79N15O16
Synonyms10Panx
Solubility≥124.2mg/mL in DMSO
SDFDownload SDF
Canonical SMILESCC(C)C(C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(CC1=CC=C(C=C1)O)C(=O)O)NC(=O)C(CC2=CC=CC=C2)NC(=O)C(C)NC(=O)C(C)NC(=O)C(CCC(=O)N)NC(=O)C(CCCN=C(N)N)NC(=O)C(CC3=CNC4=CC=CC=C43)N
运输条件试用装:蓝冰运输。 其他可选规格:常温运输或根据您的要求用蓝冰运输。
一般建议为了使其更好的溶解,请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。

试验操作

细胞实验[1]:

细胞系

人红细胞(RBCs)

溶解方法

在无菌水中的溶解度>10 mM。原液应分装,在-80℃以下可储存几个月。

反应条件

200 μM

应用

10Panx可以减少90.9±15.5%的pO2降低诱导的ATP的释放,而不影响 ILO诱导的ATP的释放。

注意事项

请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的,属于正常现象。

References:

[1] Sridharan M, Adderley S, Bowles E, et al. Pannexin 1 (PANX1) is a Conduit for ATP Released from Human Red Blood Cells (RBCs) Exposed to Low Oxygen Tension. The FASEB Journal, 2010.

生物活性

描述 10Panx(Panx-1模拟抑制肽)是pannexin-1间隙连接的阻断剂。
靶点 Gap Channels          
IC50            

质量控制

化学结构

10Panx

相关生物数据

10Panx