1,4-PBIT (dihydrobromide)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
1,4-PBIT (dihydrobromide) is a potent inhibitor of iNOS and nNOS with Ki values of 7.4 and 16 nM, respectively [1].
Nitric oxide (NO) is an endogenously produced inorganic free radical gas which has been implicated in blood pressure homeostasis, platelet aggregation, neurotransmission, and immunological defense mechanisms. NO is synthesized by three isoforms of nitric oxide synthase (NOS): nNOS, eNOS and iNOS [1].
1,4-PBIT, also known as S,S’-(1,4-Phenylenebis(1,2-ethanediyl))bisisothiourea, is a potent and selective iNOS and nNOS inhibitor. 1,4-PBIT inhibited purified human iNOS, eNOS and nNOS with Ki values of 7.4 nM, 360 nM and 16 nM, respectively. In DLD-1 cells, 1,4-PBIT inhibited human iNOS with IC50 value of 30 μM, presumably to poor membrane permeability [1].
In anesthetized rats, 1,4-PBIT (10 mg/kg, given 1 h after LPS administration), 1,4-PBIT effectively reversed the systemic hypotension, reduced the exhaled NO concentration and prevented acute lung injury. 1,4-PBIT also significantly depressed LPS-induced mRNA expressions of iNOS and IL-1β[2].
References:
[1]. Garvey EP, Oplinger JA, Tanoury GJ, et al. Potent and selective inhibition of human nitric oxide synthases. Inhibition by non-amino acid isothioureas. J Biol Chem. 1994 Oct 28;269(43):26669-76.
[2]. Wang D, Wei J, Hsu K, et al. Effects of nitric oxide synthase inhibitors on systemic hypotension, cytokines and inducible nitric oxide synthase expression and lung injury following endotoxin administration in rats. J Biomed Sci. 1999 Jan;6(1):28-35.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 444.2 |
Cas No. | 157254-60-9 |
Formula | C12H18N4S2·2HBr |
Solubility | ≤20mg/ml in DMSO;20mg/ml in dimethyl formamide |
Chemical Name | S,S'-1,4-phenylene-bis(1,2-ethanediyl)bis-isothiourea, dihydrobromide |
SDF | Download SDF |
Canonical SMILES | N/C(SCCC1=CC=C(CCS/C(N)=N/[H])C=C1)=N/[H].Br.Br |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |